摘要
目的:观察重组人粒细胞刺激因子(rh G-CSF)对肝纤维化大鼠的修复作用。方法:选取清洁级Wistar大鼠30只分为3组,即正常组、肝纤维化模型组(等量生理盐水)、实验组(rh G-CSF 200μg/kg·d),每组各10只,除正常组外均采用四氯化碳(CCl4)综合法建立肝纤维化模型,造模成功后,皮下注射相应药物1周,处死大鼠后,留取血清、肝组织,检测丙氨酸氨基转移酶(ALT)、白蛋白(ALB)、总胆红素(TBIL)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、肌酸激酶(CK)、透明质酸(HA)、层粘连蛋白(LN)、羟脯氨酸(Hyp)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)等肝功能及肝纤维化指标的变化,采用HE染色及Masson染色观察各组大鼠肝组织病理变化情况。结果:1与对照组相比,模型组、实验组大鼠血清ALT、AST、TBIL均上升(P<0.05),ALB均降低(P<0.05);与模型组对比,实验组ALT、AST、TBIL降低,ALB升高(P<0.05);2与对照组相比,模型组、实验组HA、LN、PCⅢ、Ⅳ-C上升(P<0.05);与模型组对比,实验组HA、LN、PCⅢ、Ⅳ-C均降低,对比差异有统计学意义(P<0.05);3与对照组相比,模型组、实验组ALP、CK上升(P<0.05);与模型组对比,实验组ALP、CK降低,对比差异有统计学意义(P<0.05);4与对照组相比,模型组、实验组Hy P上升(P<0.05);与模型组对比,实验组Hy P降低,对比差异有统计学意义(P<0.05)。结论:rh G-CSF可促进肝纤维化大鼠肝功能修复,减轻肝纤维化程度。
Objective： To observe the repair effect of recombinant human granulocyte colony-stimulating factor （rhG-CSF） in rats with fibrosis. Methods： Thirty clean Wistar rats were divided into 3 groups, namely the normal group, liver fibrosis model group （equivalent normal saline） and the experimental group （rhG-CSF 200 g/kg· d） with 10 rats in each. Except the normal group, the other rats were used for establishing liver fibrosis model with carbon tetrachloride （CCl4） comprehensive method. After successful modeling, the rats were treated with subcutaneous injection of corresponding drugs for 1 week. After the rats were killed, the serum and liver tissues were collected. The changes of alanine aminotransferase （ALT）, albumin （ALB）, total bilirubin （TBIL）, aspartate aminotransferase （AST）, alkaline phosphatase （ALP）, creatine kinase （CK）, hyaluronic acid （HA）, laminin （LN）, hydroxyproline （Hyp）, type III procollagen （PC III）, type IV collagen （IV-C） and other liver function and liver fibrosis indexes were detected. The pathological changes of liver tissues in rats were observed by HE staining and Masson staining. Results： OCompared with the control group, ALT, AST and TBIL in the model group and the experimental group were higher （P〈0.05） while ALB was lower （P〈0.05）; Compared with the model group, ALT, AST and TBIL in the experimental group were lower while ALB was higher （P〈0.05）; ①Compared with the control group, HA, LN, PC III and IV -C in the model group and the experimental group were higher （P〈0.05）; Compared with the model group, HA, LN, PC III and IV -C in the experimental group were lower （P〈0.05）; ②）Compared with the control group, ALP and CK in model group and experimental group were higher （P〈0.05）; Compared with the model group, CK and ALP in the experimental group were lower （P〈0. 05）; ③Compared with the control group, HyP in the model group and experimental group were higher （P〈0.05）; C
作者
林建辉
陈怡
陈达
李芹
刘豫瑞
LIN Jian-hui CHEN Yi CHEN Da LI Qin LIU Yu-rui(Department of Liver mech＇cine, The Affiliated Infectious Diseases Hospital, Fujian Medical University, Fuzhou, Fujian, 350001, China Liver Diseases Research Center, the First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, China)
出处
《现代生物医学进展》
CAS
2016年第36期7011-7014,7094,共5页
Progress in Modern Biomedicine
基金
福州市科学技术局基金项目(2014-S-139-10)
国家十二五重大专项子课题(2013ZX10005002-001-002)
关键词
重组人粒细胞刺激因子
肝纤维化
肝功能
修复
Recombinant human granulocyte colony-stimulating factor
Liver fibrosis
Liver function
Repair
