摘要
目的:探索大鼠骨髓间质干细胞(MSC),在同种异体移植后受体大鼠肝脏的定居分布情况. 方法:以绿色荧光蛋白标记从大鼠骨髓中分离培养的MSC,分别从大鼠尾静脉和门静脉注入同种异体正常和肝脏损伤大鼠体内,于移植后的第7 d,分别完整取肝,通过荧光检测和常规病理、免疫组织化学检查,研究MSC在大鼠肝脏内定居分布情况. 结果:经不同途径移植MSC,在受体大鼠肝脏内均有定居.在肝脏受损的大鼠肝内,其已成功地分化为成熟的肝细胞和卵圆细胞,并能长期存活,在肝脏未受损的大鼠,MSC仅少量分化为卵圆细胞,所移植的MSC 定居于肝脏和是否分化为成熟肝细胞、卵圆细胞与其移植途径无明显关系,与肝脏是否受损伤相关. 结论:植入MSC是肝脏卵圆细胞的细胞来源,并且可以分化为成熟的肝细胞.干细胞定居于肝脏数量与肝脏是否受损伤关系密切,与移植途径无关.
AIM: To study the distribution and amount of the mesen-chymal stem cells (MSCs) homing to the liver during al-lografting in rats. METHODS: MSCs were isolated from the marrow of the rats using green fluorescent protein and cultivated in vitro. MSCs transduced with GFP retroviral vector were subsequently infused into the tail or portal veins of different rats with homogeneity, some of whichhad been treated with carbon tetrachloride to induce centrolobular liver necrosis while some of which were healthy. At the 7th day after transplantation, the whole livers were collected. The homing status and distribution of MSCs were studied by fluorescence detection, routine pathological examination and immunohistochemstry. RESULTS: The transplanted cells could be found in all the livers of rats. Furthermore, MSCs resided in the damaged liver of the rats and differentiated into oval cells and mature hepatocytes. Only a few MSCs differentiated into oval cells in the healthy livers. The homing status and differentiation of MSCs were closely related to whether the liver was damaged, but not to the approach of transplantation. CONCLUSION: The allografted MSCs can successfully reside in the damaged liver of rats and differentiate to mature hepatocytes. The homing status has no relation with the approach of transplantation.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第10期1198-1201,共4页
World Chinese Journal of Digestology
基金
广东省自然科学基金资助项目
No.2001
01059
2002.020097~~
