摘要
目的粒细胞集落刺激因子(G CSF)单用或合用重组人生长激素(rhGH)动员急性心肌梗死大鼠自体骨髓干细胞,观察梗死区心肌和血管再生状况。方法液氮冷冻法制备心肌梗死模型。动物随机分为正常动员组(N,n=8)、假手术组(SO,n=6)、梗死组(MI,n=8)、G CSF治疗组(G,n=8)、rhGH治疗组(GH,n=8)和G CSF+rhGH治疗组(GG,n=8)。计数动员前后白细胞计数(WBC)及单个核细胞百分比(MNC%);术后4周处死动物,取心脏称重,行苏木素伊红(HE)染色及免疫组化染色。结果1N组及G组动员后WBC和MNC%较动员前均明显增加(P均<0.01)。MI组动员后WBC明显高于动员前(P<0.01),但MNC%与动员前比较差异无显著性(P>0.05)。G组动员后WBC和MNC%均显著高于MI组动员后(P均<0.05)。2MI、G、GH、GG4组间梗死面积无明显差别(P>0.05)。3GH、GG组动物处死时体重和心脏重量均明显高于SO、MI和G组(P均<0.05)。GG组心脏重量/体重比高于SO、MI和G组(P<0.05)。4G、GH和GG组毛细血管计数明显多于MI、SO组,GG组多于G、GH组(P均<0.01)。5G、GH和GG组均可见BrdU(+)新生细胞,部分形成新生毛细血管;G、GG组还可见新生心肌样细胞。结论1G CSF可动员正常和心肌梗死后大鼠自体骨髓干细胞到外周血循环并进入梗死区,使大鼠梗死区心肌样细胞及毛细血管再生。2rhGH可促进梗死区毛细血管新生,但不再生心肌样细胞。3G CSF联合rhGH治疗使大鼠梗死区毛细血管密度明显增加,提示两药合用有相加作用。
Objective To observe the regeneration cardiomyocytes and neovascularization after mobilizing autologous bone marrow stem cells by granulocyte colony stimulating factor （G -CSF） alone or G- CSF combined with recombinant human growth hormone （rhGH） in Wistar rats with acute myocardial infarction （AMI）. Methods AMI rat model was reproduced by liquid nitrogen cryoinjury. The rats were randomly divided into six groups： mobilization group （N group, n = 8）, sham operation group （SO group, n：6）, myocardial infarction group （MI group, n=8）, G -CSF group （G group, n=8）, rhGH group （GH group, n=8） and G -CSF combined with rhGH group （GG group, n=8）. White blood cell （WBC） count and mononuclear cells proportion （MNC%） in peripheral blood were determined with ABX blood cell analyzer to estimate bone marrow stem cells mobilization. Four weeks after intervention, the rats were sacrificed, their respective body and heart weight were obtained, and the hearts were harvested for hematoxylin and eosin （HE） and immunohistochemical examination. Results （1）Comparing with baseline values, after 6 days administration of G- CSF, the WBC and MNC% increased in N and G groups （both P〈0. 01）; WBC increased （P 〈0. 01） but no difference of MNC% in MI group （P〉 0. 05）; WBC and MNC% were significantly higher in G group than those in MI group （all P〈0.05）. （2）Body and heart weights in GH and GG groups were higher than those in SO, MI and G groups respectively （all P〈0.05）. The ratio of heart and body weight was higher in GC group than that in MI,G and SO groups （P〈0. 05）. （3）There were no significant differences in infarct size among MI, G, GH, and GG groups （P〉0. 05）. （4）The capillary densities were higher in G, GH and GG groups than those in MI and SO groups; the density in GG group was higher than that in G and GH groups （all P〈0.01）. （5）BrdU positively stained neonatal cells were observed in G, GH and GG groups. Of them some
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2006年第8期494-497,F0003,共5页
Chinese Critical Care Medicine
基金
天津市卫生局科技基金资助项目(02KZ40)
关键词
心肌梗死
急性
骨髓干细胞动员
粒细胞集落刺激因子
生长激素
重组
心肌再生
血管新生
acute myocardial infarction
bone marrow stem cells mobilization
granulocyte colony stimulating factor
growth hormone
cardiomyocyte regeneration
neovascularization
