摘要
目的:观察运动训练对脑出血大鼠神经细胞凋亡基因caspase-3及其蛋白表达的影响。方法:实验动物用健康雄性SD大鼠120只。随机分为3组,实验组(出血后运动,40只)、对照组(出血后不运动,40只)、假手术组(无出血不运动,40只)。各组又分为术后7d,14d,21d,28d共4个时相点,每个时相点5只用于免疫组化检测,5只用于RT-PCR和Western印迹法检测。实验组于术后24h开始跑笼训练,其余大鼠在标准笼内自由活动。结果:①免疫组化结果:caspase-3阳性细胞表达为细胞胞浆着棕黄色,阳性细胞主要分布于血肿周围和大脑皮质的神经元。实验组随运动训练时间延长似乎有表达上调趋势,28d时表达虽有下降迹象,但仍处于较高水平。对照组表达水平较实验组更明显,但两组差异无显著性意义(P>0.05),实验组和对照组与假手术组相比差异有非常显著性意义(P<0.01)。②Western blotting和RT-PCR结果与免疫组化一致。结论:caspase-3参与了脑出血后细胞凋亡的发生,但运动训练对caspase-3基因及其蛋白表达的抑制作用不明显。
Objective：To investigate the exercise effects on expression of easpase-3 of neuronal cells apoptosis after ICH in rats. Method： A total of 120 male SD rats（weight, 270 to 300g） were divided into three groups, trial group （ICH-induction and exercise group, n=40）, control group （ICH-induction group, n=40） and sham operated group （no ICH without exercise, n=40）. The rats brains were removed at 7d,14d,21d,28d after ICH. The activation of caspase-3 was measured by immunohistochemistry, Western blotting and RT-PCR. Result：①caspase-3-positive, appeared around the periphery of the hematoma and cortex. The number of caspase-3-positive cells was nearly zero in the sham-operation group. Although the expression of caspase-3 had a up-regulation trend in trial group and control group, but there was no difference between the two groups. There was a significant difference compared with sham operated group （P〈0.05）.②The result of Western blotting , RT-PCR for caspase-3 mRNA showed the continuity with that of immunohistochemistry. Conclusion：Caspase-3 participated in neural apoptosis after ICH, but exercise training （cage-running） did not depress clearly the expression of caspase-3 and caspase-3 mRNA.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2008年第7期601-604,I0001,共5页
Chinese Journal of Rehabilitation Medicine
