摘要
目的:观察脑红蛋白在实验性脑出血模型大鼠脑组织内表达水平的动态变化。方法:实验于2004-10/2005-10在解放军沈阳军区总医院动物实验科和神经中心实验室完成。66只大鼠随机分为3组:正常对照组6只,脑出血组30只和假手术组30只。脑出血组和假手术组再根据动物模型建立后1,6,24,48和72h5个时间点随机分为5个亚组,每个亚组6只大鼠。采用立体定向自体血注入大鼠尾状核建立脑出血模型(假手术组注入生理盐水),用Westernblot和免疫组织化学法检测脑出血后不同时间脑组织内脑红蛋白的表达。结果:脑出血组有3只大鼠术后死亡,有4只大鼠术后模型制备失败,均予以剔除并随机补充,66只大鼠最终纳入分析。Westernblot结果显示脑出血组术后1h血肿周围脑组织内脑红蛋白相对表达水平开始增高(0.45±0.03),48h达到高峰(0.83±0.05),72h开始下降(0.55±0.11),均明显高于假手术组(0.31±0.06,0.31±0.01,0.33±0.04)和正常对照组(0.32±0.07)。免疫组化染色显示在血肿周围脑红蛋白表达比其他脑区明显增多。结论:脑出血后脑内脑红蛋白表达上调,提示脑红蛋白可能对脑出血继发神经损伤起重要的保护作用。
AIM： To observe the dynamic changes of the expression of neuroglobin in brain tissue of rats with intracerebral hemorrhage. METHODS： The experiment was carried out in the Department of Animal Experiment and Central Laboratory of Neurology of the General Hospital of Shenyang Military Area Command of Chinese PLA from October 2004 to October 2005. Sixty-six rats were randomly divided into normal control group （n =6）, sham-operated group （n =30） and intracerebral hemorrhage group （n=30）. The sham-operated group and intracerebral hemorrhage group were divided into 5 subgroups respectively at 1, 6, 24, 48 and 72 hours after the establishment of animal model, every subgroup included 6 rats. Intracerebral hemorrhage was induced by stereotaxic injection of autologous blood into left caudate nucleus of the rats （normal saline was injected in sham-operated group）. Both Western blot and immunohistochemistry were used to detect the expression of neuroglobin in brain tissue at different times after intracerebral hemorrhage. RESULTS： In the intracerebral hemorrhage group, 3 rats died postoperatively, and 4 rats failed in the model establishment, they were replaced by other randomly selected onss, finally 66 rats were involved in the analysis of results. Western blot analysis showed that the relative expression level of neuroglobin in brain tissue in the intracerebral hemorrhage group began to increase at 1 hour （0.45±0.03）, reached its peak value at 48 hours （0.83±0.05）, and began to decrease at 72 hours （0.55±0.11）, which were all obviously higher than those in the sham- operated group （0.31±0.06, 0.31±0.01, 0.33＋0.04） and that in the normal control group （0.32±0.07）. The immunohistochemistry showed that the expression of neuroglobin in perihematoma was obviously increased as compared in other brain areas. CONCLUSION： The expression of neuroglobin in brain tissue is upregulated after intracerebral hemorrhage, it is indicated that neuroglobin may play an important protective e
出处
《中国临床康复》
CSCD
北大核心
2006年第6期97-99,F0003,共4页
Chinese Journal of Clinical Rehabilitation
