摘要
目的观察大鼠脑出血后神经肽Y的表达与脑出血后脑含水量变化的关系,以及重组活化凝血因子Ⅶ对其的影响。方法通过采用未抗凝新鲜自体股动脉血注入大鼠尾状核建立脑出血动物模型,用放射免疫法测定脑出血后6h和1、3及7d神经肽Y的变化,用干重-湿重法测定脑出血后脑含水量的变化,并与神经肽Y进行相关分析。结果与对照组比较,血肿周围组织中神经肽Y水平在脑出血后1和3d时显著升高(P<0.05),7d时降至正常。血肿周围组织含水量在脑出血后6h轻度增加,1d时显著增加(P<0.01),3d时达高峰(P<0.01),以后逐渐下降,7d时基本恢复正常水平;重组活化凝血因子Ⅶ组脑含水量及神经肽Y含量均明显减少。结论神经肽Y可能参与实验性脑出血后血肿周围水肿的形成;重组活化凝血因子Ⅶ对二者有抑制作用,重组活化凝血因子Ⅶ可能有助于减轻脑出血后脑水肿的形成。
ObjectiveTo investigate dynamic changes of water content and neuropeptide Y (NPY) in intracerebral hemorrhagic (ICH) rats and the role that neuropeptide Y plays in cerebral edema after hemorrage, and to evaluate the effect of recombinant activated factor Ⅶ (rF Ⅶa) in reducing the content of cerebral edema and neuropeptide Y.MethodsAnimal model was made by injecting self arterial blood into the caudate nucleus of rats. 75 rats were randomly assigned to control group, cerebral hemorrhage group and rFⅦa treated group. After the injection, the rats were sacrificed at 6 hours, 1, 3 and 7 days respectively. Brain water content was determined by wet-dry weight, as the expression neuropeptide Y was detected by radioimmunoassay method over a time course ranging from 6 hours to 7 days. ResultsBrain water content and neuropeptide Y increased after 6 hours of cerebral hemorrhage and reached its climax at the 3 days (P <0.01), then decreased from the 5 to the 7 days; Administration of rFⅦa could reduce the content of brain edema and neuropeptide Y.ConclusionsRecombinant Activated Factor Ⅶ can reduce neuropeptide Y expression, thus can reduce cerebral edema after ICH. The rFⅦa might be available for the therapy of cerebral edema after ICH.
出处
《中国医学工程》
2009年第5期332-334,339,共4页
China Medical Engineering
关键词
神经病学
脑出血
神经肽Y
抑肽酶
脑水肿
intracerebral hemorrhage
neuropeptide Y
cerebral edema
recombinant activated factor Ⅶ
rats
