摘要
目的观察运动训练对脑出血大鼠神经细胞凋亡基因Bcl-2、Bax及其蛋白表达的的影响。方法共选取健康雄性sD大鼠120只，将其随机分为3组。实验组大鼠采用胶原酶诱导法制作大鼠脑出血模型；对照组大鼠手术操作同实验组，但术后不给予运动训练；假手术组大鼠正常饲养。实验组大鼠于术后24h开始跑笼训练，其余大鼠则在标准笼内自由活动。各组大鼠分别于术后第7，14，21及28天时各取5只用于免疫组化检测，另取5只用于RT-PCR及Westernblotting检测。结果①免疫组化结果：大鼠Bcl-2、Ba~阳性细胞胞浆均呈棕黄色，阳性神经元主要分布于血肿周围及大脑皮质区域。实验组大鼠Bcl-2表达水平于术后第21天时开始上升，与假手术组比较，差异有统计学意义（P〈0．01），于术后28d时达到峰值，此时与对照组及假手术组比较，差异均有统计学意义（P〈0．01）。实验组大鼠Bax表达水平于术后7d时开始下降，术后14～21d下降显著，术后28d时虽有回升趋势，但与对照组和假手术组比较，差异仍有统计学意义（P〈0．05）。②Westernblotting及RT．PCR检测结果：各组大鼠检测结果与免疫组化检测结果基本-致，但实验组大鼠Bcl-2mRNA和BaxmRNA表达高峰时间均较相应蛋白表达高峰时间提前。结论运动训练可上调脑出血大鼠Bcl-2基因及其蛋白表达，下调Bax基因及其蛋白表达，从而抑制脑出血后细胞凋亡，促进功能恢复。
Objective To investigate the effects of therapeutic exercise （cage running） on the expression of Bcl-2 and Bax in neuronal cells after cerebral hemorrhage （ICH） in rats. Methods A total of 120 male Sprague- Dawley rats weighing 270 to 300 g were divided into a trial group （ ICH and exercise group, n = 40） , a control group （ ICH only, n =40） and a sham-operated group （ sham ICH operation and no exercise, n =40）. The brains were re- moved at 7, 14, 21 and 28 days after ICH. The activation of Bcl-2 and Bax was measured by immunohistochemical techniques, Western blotting and reverse transcriptase polymerase chain reaction （RT-PCR）. Results （ 1 ） Bel-2- positive and Bax-positive cells appeared in tissues surrounding the hematoma in the cortex. The number of Bcl-2-po- sotive and Bax-positive cells was nearly zero in the sham-operated group. In the trial group, the number of Bcl-2-pos- itive ceils increased significantly from the 21st to the 28th day. Bax-positive cells decreased from the 7th to the 28th day after ICH when compared with the control group and the sham-operated group. （2） The expression of Bcl- 2mRNA and Bax-mRNA as shown by Western blotting and RT-PCR methods was synchronous with the immunohisto- chemistry results. The peaks of Bcl-2mRNA and BaxmRNA expression were a little earlier than those of Bcl-2 and Bax proteins. Conclusion Exercise can mitigate neuronal cell apoptosis by upregulating the expression of Bcl-2 and Bcl-2 mRNA, and downregulating the expression of Bax and Bax mRNA.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2008年第8期509-513,共5页
Chinese Journal of Physical Medicine and Rehabilitation
