摘要
目的探讨VEGF-C和COX-2与结肠癌发病及淋巴结转移的关系。方法选择62例结肠癌(肠癌组)、22例结肠腺瘤(腺瘤组)和22例正常结肠黏膜(正常组)标本,采用原位分子杂交法检测环氧化酶2(COX-2)mRNA,以MaxVisionTM快捷免疫组化法检测血管内皮生长因子C(VEGF-C)及血管内皮细胞表面抗原(CD34)表达,光镜下记录微血管计数(MVD)。结果VEGF-C在肠癌组的阳性率为74.19%(46/62)明显高于腺瘤组(36.36%、8/22)和正常组(36.36%、8/22)(P<0.01)。VEGF-C表达阳性者中高表达占82.61%,随着肿瘤体积的增大、组织分化程度的降低阳性表达率也有升高,淋巴结有转移组表达阳性率略高于无转移组。COX-2 mRNA在肠癌组、腺瘤组的阳性表达率明显高于正常组(74.19% vs .36.36%,P<0.01;72.73% vs .36.36%,P<0.05);COX-2 mRNA在高分化组阳性率高于低分化组(P<0.05);VEGF-C和COX-2的表达呈正相关(P<0.01);肠癌组平均MVD值明显高于腺瘤组和正常组(P<0.01)。进展期癌组的MVD高于早期癌组(P<0.01);淋巴结有转移组MVD高于无转移组(P<0.01);有血管侵犯组高于无血管侵犯组(P<0.05)。结论COX-2通过上调VEGF-C的表达在结肠癌肿瘤血管形成及淋巴结转移中起促进作用。
Objective To study the expressions of vascular endotheliol growth factor C（VEGF- C）, cyclooxygenase-2（COX-2） mRNA and microvessel density （MVD） in colon carcinoma and their significance in the angiogenesis, lymph node metastasis and clinical pathologic characteristics. Methods Expression of COX-2 mRNA was evaluated by in situ hybridization method and that of VEGF-C and CD34 was detected immunohistochemically by Maxvision^TM method in 62 cases of colon carcinoma, 22 cases of colon adenoma and 22 cases normal colon tissues. Results The percentage of positive VEGF-C was 74.19 % in 62 cases of colon carcinoma, which was significantly higher than that of colon adenoma and normal colon tissues （P〈0. 01）. Expressions of COX-2 mRNA were detected in a significantly greater proportion of colon carcinoma and adenoma samples than those in the normal colon tissues[-74.19% vs. 36.36%（P〈0. 01） and 72.73% vs. 36.36%（P〈0. 05）]. The expression of MVD was significantly greater in colon carcinoma samples than that in adenoma and normal tissues of colon （P〈0. 01）. In 62 cases with colon carcinoma, the percentage of VEGF-C expression was 82. 61%, which was positively correlated with the expressions of VEGF-C and COX-2 mRNA （P〈 0. 01）. The positive expression of COX-2 mRNA in high differentiation group was significantly higher than that in low differentiation group （P〈 0. 05 ）. The expression of MVD in advanced carcinoma group was significantly greater than that in early carcinoma group （P〈0. 01）. MVD in the patients with lymphatic metastasis and angiogenesis infiltration was higher than that in those without lymphatic metastasis and angiogenesis infiltration （P〈0. 01 or P〈0. 05）. Conclusion COX-2 may play an important role in tumor cell proliferation and lymphatic metastasis by upregulating VEGF-C in colon carcinoma.
出处
《江苏医药》
CAS
CSCD
北大核心
2008年第7期655-658,I0003,共5页
Jiangsu Medical Journal
基金
连云港市科技发展项目资助(SH0601)
关键词
结肠癌
血管内皮生长因子C
环氧化酶2
微血管密度
Colon carcinoma
Vascular endoehelial growth factor C
Cyclooxygenase-2
Microvessel density
