摘要
在实验室模拟条件下分别采用动、静态生物慢性实验的方法,研究了不同浓度的四溴双-酚A(Tetrabromobisphenol A,TBBPA)对颤蚓(Monopylephorus limosus)体内抗氧化酶系统及相Ⅱ结合酶系统活性的影响以及颤蚓暴露于单一浓度的TBBPA后,其体内抗氧化酶系统的活性随时间的响应.结果表明,暴露8 d后,颤蚓体内SOD的活性发生显著变化,变化趋势明显分为升高、降低、再升高3阶段,在0.05 mg/L时SOD的活性被最大诱导(p<0.01),达到对照组的7.8倍,各浓度组SOD的活性均显著高于对照组,由对照组的1.5倍到对照组的7.8倍;同时,CAT的活性变化则呈升高、降低、再升高、再降低4阶段,0.5 mg/L时其活性达到最大值(p<0.01),除0.005 mg/L和0.25 mg/L浓度组CAT活性受抑制外,其余浓度组CAT的活性均高于对照组,由对照组的1.1倍到1.9倍.染毒浓度为0.25 mg/L时,GST的活性达到最大诱导(p<0.01),其活性先缓慢上升后下降,并且各处理组GST的活性均显著高于对照组(p<0.05).在单一浓度TBBPA污染暴露的10 d时间内,颤蚓体内SOD的活性变化趋势形成了一个变形的'M'形曲线,在第3 d时其活性达到最大诱导,而CAT活性的变化则表现为一个不规则的'N'形曲线,在第5 d时其活性达到最大诱导,并且SOD的活性比CAT的活性受时间的影响小,相对来说更稳定.可见,SOD与GST的活性变化似乎更能反映出TBBPA对颤蚓的污染效应及其毒性作用,而SOD的活性变化似乎更为灵敏,但是二者能否作为指示TBBPA污染的生物标志物尚需进一步研究.
Using the indoor simulating method of dynamic and static exposure respectively, the toxic effects of TBBPA on the antioxidant enzyme defense systems and Glutathione-S-transferase (GST) activity of tubifex Monopylephorus limosus were examined. The activity of superoxide dismutase (SOD) and catalase (CAT) with time was also examined. The results showed that after an 8 d exposure, the SOD activity was enhanced at first and then inhibited gradually, at last enhanced again .The highest activity of SOD (p 〈 0.01 ) was examined under 0.05 mg/L concentration of TBBPA.And the activity of SOD was much higher than that of control (1.5-7.8 times more than that of the control). The activity of CAT showed a tendency of induction firstly and then inhibition, then induction again and at last inhibition, reached the highest value under 0.5 mg/L of TBBPA.Furthermore, the CAT activity was higher than that of the control (1.1-1.9 times more than that of the control) except that under 0.005 mg/L and 0.25 mg/L of TBBPA. Moreover, the highest activity of GST (p 〈 0.01 ) was observed under 0.25 mg/L of TBBPA.The activity of GST was enhanced gradually at first and then inhibited. As the same as SOD, the activity of GST was induced significantly (p 〈 0.05) .The changes in the SOD activity showed an "M" trend,while that in the CAT activity showed an "N" trend.And the activity of SOD is steadier than that of CAT. Thus, changes in the activity of SOD and GST, especially SOD, can better reflect the toxic effects of pollutants on tubifex.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2008年第7期2012-2017,共6页
Environmental Science
基金
教育部培育基金项目
国家重点基础研究发展规划(973)项目(2004CB418503)