摘要
在体内实验条件下,测定了鲤鱼肝脏过氧化氢酶的活性.实验结果表明,过氧化氢酶的紫外分光光度法是可靠的.活性抑制结果表明,对-二硝基苯(p-DNB)对鲤鱼肝脏过氧化氢酶均有明显的抑制作用,EC_(50)值为0.0807mg·1^(-1).鲤鱼肝脏过氧化氢酶可作为一项指标用于生态毒理学研究.
Liver catalase (Ct) activities of carp were measured by ultraviolet spectropho-tometry and the results were reliable. The effect of p-DNB on Ct of carp liver were studied in Vivo. The results showed that p-DNB exhibited fairly strong inhibition to Ct of carp livers. A satisfactory straight line was obtained by plotting the probits of inhibition percentage of Ct against the logarithmic concentration of toxicants, The concentration causing 50% inhibtion of p-DNB to Ct carp liver was 0. 087mg·I-1. It was proposed that the effect of toxicant on Ct could be used as an index of ecotoxicolgical assessment.
出处
《环境化学》
CAS
CSCD
北大核心
1997年第1期73-76,共4页
Environmental Chemistry
基金
国家自然科学基金资助课题
