摘要
目的研究存活素、P53蛋白、增殖细胞核抗原(PCNA)在胆囊良恶性病变组织中的表达及其意义,并探讨其相关性。方法用免疫组化SP法染色,对68例胆囊良恶性病变组织的石蜡标本(A组:胆囊癌37例;B组:胆囊腺瘤9例;C组:胆囊腺肌症12例;D组:慢性胆囊炎10例)进行存活素、P53蛋白、PCNA表达的检测分析。结果存活素、P53蛋白、PC-NA在A组细胞中阳性表达率(75.7%、54.1%、97.3%)最高,明显高于D组(20.0%、0、40.0%);存活素、P53蛋白、PCNA的表达分别呈正相关(r1=0.58,r2=0.42,r3=0.41,P均<0.01)。结论存活素与突变型P53蛋白在胆囊癌的发生发展中可能起协同作用,野生型P53可能对存活素的表达起负反馈调节作用,二者可作为靶点用于肿瘤靶向治疗的研究。
Objective To analyze the expression and clinicopathological relationship of survivin, P53 and PCNA in benign and malign gallbladder diseases. Methods The immuno- histochemistry was used to detect the expression and clinicopathological relationship of survivin, P53 and PCNA in benign and malign gallbladder diseases （including 37 cases of gallbladder carcinoma, 9 cases of gallbladder adenoma and 10 cases of chronic cholecystitis, 12 cases of cholecystoadenomyomatosis）. Results Positive immunoreactivity was detected in a proportion of 75.7% for survivin, 54. 1% for P53, 97. 3% for PCNA in the gallbladder carcinoma, respectively. And their expressions in malign lesions and in benign diseases were significantly different. The expression of survivin correlated positively with P53 expression（r = 0. 58, P〈0. 01） and inversely with PCNA （r = 0. 42, P〈0. 01）. Significant correlation was found between the expression of PCNA and P53 （r = 0. 41 ,P〈0. 01）. Conclusion The over-expression of survivin and P53 played a synergetic role in the carcinogenesis and progression of gallbladder carcinoma. Survivin and P53 may be identified as a potential therapeutic target in gallbladder carcinoma.
出处
《华中医学杂志》
2008年第3期215-216,共2页
Central China Medical Journal
