摘要
目的 :探讨Survivin基因在原发性胆囊癌 (PGC)中的表达情况 ,以及与P5 3,PCNA蛋白表达的相互关系。方法 :逆转录聚合酶链反应 (RT PCR)法检测 9例PGC新鲜癌组织SurvivinmRNA表达 ,对 38例PGC石蜡包埋组织应用免疫组织化学法检测Survivin ,P5 3和PCNA蛋白的表达 ,并将结果进行相关分析。结果 :9例PGC新鲜癌组织中 3例有SurvivinmRNA表达。 2 1例 (5 5 .2 % )PGCSurvivin蛋白表达阳性 ,而 12例胆囊腺瘤中有 2例表达 ,2 0例癌旁正常组织中则无表达。PGCSurvivin蛋白表达阳性率显著高于胆囊良性病变和正常胆囊组织 (P <0 .0 1)。Survivin蛋白在PGC中的表达与PCNA指数相关 ,与P5 3蛋白表达、PGC病理分级和Neiv分期无显著相关。结论 :Survivin基因在PGC中存在高表达 ,Survivin基因表达上调通过不同的机制影响PGC细胞的凋亡和增殖 ,在PGC发生、发展过程中起作用。
Objective:To study the expression of survivin and its correlation with expression of P53,PCNA protein levels in primary gallbladder carcinoma(PGC).Methods:Expression of the survivin mRNA was evaluated with reverse transcritase polymerase chain reaction(RT-PCR) in 9 fresh tumor samples of PGC. Inmmunohisochemical assay was used to detect the expression of survivin,P53 and PCNA protein in 38 paraffin embedded sections.Results:Survivin mRNA was observed in 3 of 9 cases of PGC with RT-PCR. Survivin protein positive rate was 55.2%,16.7% and 0,respectively in PGC,adenoma and normal tissue. The positive rate was significantly higher in malignant lesion than that in benign diseases(P<0.01). Expression of survivin is correlated with PCNA index,but not with expression of P53,the pathologic classification and Neiv's staging of PGC.Conclusion:The up-regulation expression of survivin is correlated with cancer cell apoptosis and proliferation. The development of PGC may be associated with survivin expression.
出处
《肝胆胰外科杂志》
CAS
2005年第1期17-19,共3页
Journal of Hepatopancreatobiliary Surgery
