摘要
目的探讨乙型肝炎病毒(HBV)前S1抗原(Pre-S1Ag)与HBV-DNA、乙型肝炎e抗原(HBeAg)的关系,了解检测Pre-S1Ag的临床意义。方法用酶联免疫吸附试验检测Pre-S1Ag、HBeAg,荧光定量聚合酶链反应检测HBV-DNA。结果(1)在205例HBV-DNA阳性人群中,Pre-S1Ag总阳性率为84.3%,HBeAg总阳性率为79.0%,两者比较差异无统计学意义(P>0.05)。(2)当HBV-DNA>5×104copy/mL时,Pre-S1Ag阳性率为88.3%,HBeAg阳性率为96.6%,两者比较差异无统计学意义(P>0.05);当HBV-DNA<5×104copy/mL时,Pre-S1Ag阳性率为78.8%,HBeAg阳性率为54.1%,两者比较差异有统计学意义(P<0.05)。(3)在HBV-DNA水平不同的两组中,HBeAg的阳性率分别为96.6%和54.1%,差异有统计学意义(P<0.01);Pre-S1Ag阳性率分别为88.3%和78.8%,差异无统计学意义(P>0.05)。结论前S1抗原与HBV-DNA有高度的相关性,可以作为HBV存在和复制的指标;前S1抗原与HBeAg联合检测对判断HBV的存在和复制更有价值。
Objective To investigate the relationship between the Pre-S1 antigen （Ag） and HBV-DNA as well as HBeAg, and to understand the clinical significance of Pre-S1 antigen. Methods ELISA was used to measure Pre- SlAg and HBeAg, while HBV-DNA was determined by applying quantitative PCR assay. Results The total positive rate of Pre-S1 antigen and HBeAg was 84.3% and 79.0% respectively in 205 patients with HBV-DNA positive. The positive rate of Pre-S1 antigen and HBeAg was 88.3% and 96.6% respectively in HBV-DNA〉5 X 10^4 copy/mL group was 88.3%, and 78.8% and 54.1% respectively in HBV-DNA〈5 X 10^4 copy/mL group. The difference of HBeAg was statistically significant between the above two groups （P〈0. 01）, whereas there wasn＇t statistical difference of Pre-S1 antigen between the two groups （P〉0.05）. Conclusion Pre-S1 Ag and HBV-DNA has high relevance. Pre-S1 Ag may be used as a marker of HBV existence and duplication. Combined detection of Pre-S1 Ag and HBV-DNA is of important value in identifying the existence and duplication of HBV.
出处
《检验医学与临床》
CAS
2008年第13期793-794,共2页
Laboratory Medicine and Clinic
