摘要
目的:通过对比检测和分析739份血清中preS1蛋白、HBeAg和HBV-DNA的检测阳性率,为临床正确选用实验室指标防治乙型肝炎提供依据。方法:应用ELISA法对739份血清进行PreS1Ag和HBV血清标志物检测,并与实时荧光定量PCR检测HBV-DNA结果进行比较。结果:所有739份血清中,PreS1Ag阳性率为34.6%(256/739),HBeAg阳性率为30.7%(227/739),HBV-DNA阳性率为58.9%(435/739)。227份HBeAg阳性血清中,PreS1Ag阳性率55.9%(127/227),HBV-DNA阳性率96.0%(218/227);512份HBeAg阴性血清中,PreS1Ag阳性率25.2%(129/512),HBV-DNA阳性率为42.4%(217/512)。HBeAg、HBV-DNA和PreS1的阳性率有显著差异(P<0.05),阳性率高低依次为HBV-DNA>PreS1>HBeAg;HBeAg阳性血清中PreS1Ag(55.9%)阳性率显著高于HBeAg阴性血清PreS1Ag(25.2%)阳性率(χ2=36.5,P<0.01);HBV-DNA阳性血清的PreS1Ag检出率58.9%(256/435)显著高于HBV-DNA阴性血清的PreS1Ag检出率18.4%(56/304)(P<0.01);PreS1抗原与HBV-DNA阳性符合率为(200/256)78.1%,阴性符合率为(248/483)51.3%。598份HBsAg阳性血清(阳性率为80.9%,598/739)中HBeAg、PreS1和HBV-DNA阳性数(率)分别是224(37.5%)、253(42.3%)、417(69.7%)。结论:PreS1Ag、HBV-DNA、HBeAg的阳性率有显著性差异(P<0.05)。作为HBV感染和复制的指标,以检测HBV-DNA最为可靠,而PreS1Ag可能较HBeAg更敏感。在HBV感染的不同时期,如何理解和使用上述指标对防治乙型肝炎有重要意义。
Objective：To compare the positive rate of HBeAg, PreslAg and HBV - DNA in 739 serum samples so as to offer the basis of selecting correctly examination indexes for the prevention and treatment of hepatitis B. Methods： The markers of PreslAg and HBV in 739 serum samples were detected by ELISA and the marker of HBV - DNA by real time fluorescence quantitative PCR（FQ PCR）. The detected results were compared each other. Results： In all samples, the positive rates of PreslAg, HBeAg and HBV - DNA were 34. 6% （435/739）, 30. 7%（227/739） and 58. 9% （435/739）, respectively. In 227 samples with positive HBeAg, the positive rates of PreslAg and HBV - DNA were 55.9% and 96.0%, respectively. In 512 samples with negative HBeAg, the positive rates of PreslAg and HBV- DNA were 25. 2% and 42.4%, respectively. The positive rate of Pres1Ag（58. 9%） in samples with positive HBV - DNA was significantly higher than that in the samples with negative HBV - DNA（18, 4 %, P〈0. 01）. The positive coincidence was 78. 1% （200/256） and the negative coincidence was 51.3% between PreslAg and HBV - DNA. In 598 serum samples with positive HBsAg（80. 9%, 598/739）, the positive rates of HBeAg, PreSlAg and HBV - DNA were 37.5%, 42. 3% and 69. 7%. Conclusion：There are significant differences of positive rate among HBeAg, PreSlAg and HBV - DNA. HBV - DNA is more accurate for the detection of hepatitis B than the other two indexes, but PreSlAg is more sensitive than HBeAg in judging the infection and replication of HBV.
出处
《西南国防医药》
CAS
2006年第6期604-606,共3页
Medical Journal of National Defending Forces in Southwest China
