摘要
研究了摇瓶中不同浓度纸浆为碳源对里氏木霉产纤维素酶的影响。结果表明:最佳的碳源质量浓度为12g/L,在此条件下第3天的滤纸酶活、CMC酶活和β-葡萄糖苷酶酶活分别为1.68、0.96和0.28U/mL。总碳源为15g/L下采用分批补料技术可以有效提高里氏木霉分泌纤维素酶。使用起始纸浆浓度为9g/L,第2、3、4天分别加入2g/L纸浆,其第3天的滤纸酶活、CMC酶活和β-葡萄糖苷酶酶活分别为2.41、0.72和0.27U/mL,较15g/L纸浆为碳源分批培养滤纸酶活提高1.8倍。
Different pulp concentration as carbon source for cellulase proauction by Trichoderma reesei was investigated. The best pulp concentration was 12 g/L. After 3 days of cultivation, the filter paper activity, CMCase activity and β - glucosidase activity were 1.68,0.96 and 0. 28 U/mL respectively. Fed-batch manner could significant increase cellulase production by Trichoderma reesei. The fed-batch manner was that initial pulp concentration was 9 g/L and 2 g/L pulp was added to the culture at second, third and fourth day. After 3 days of cultivation, the filter paper activity, CMCase activity and β - glucosidase activity became 2.41, 0. 72 and 0.27 U/mL respectively. Compared with batch culture used 15 g/L pulp, the filter paper activity increased 1.8 times.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期57-60,共4页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
江苏省高技术研究计划资助项目(BG2005327)
