摘要
为了提高分批补料制备纤维素酶的生产能力,本研究采用间歇出酶的方法,定期将部分酶蛋白从培养液中分离出来,以达到高效产酶的目的。在以里氏木霉为产酶菌、以纸浆为碳源的分批补料产酶过程中,从第4天开始间歇出酶。当每天取出10.0%~20.0%的酶液时,总滤纸酶活和总β-葡萄糖苷酶活分别提高26.3%~40.3%和69.8%~108.9%。为避免感染杂菌,菌丝体也可以不回收;当每天取出15.0%的酶液和菌丝体时,总滤纸酶活提高42.9%,总β-葡萄糖苷酶活提高84.3%,菌丝体质量浓度维持在4~5 g/L。结果表明:只要菌丝体浓度维持在合理范围内,间歇出酶可以保护已产出的酶蛋白,降低培养液中的产物浓度,从而大幅度提高产酶能力。
Periodical enzyme recovery in a fed-batch cellulase production was investigated to improve the productivity.In the fed-batch cellulase production from pulp by Trichoderma reesei,periodical enzyme recovery was started on the fourth day.When 10. 0% to 20. 0% of cellulase was recovered daily,the total filter paper activity and β-glucosidase activity increased by 26. 3% to 40. 3% and 69. 8% to 108. 9%,respectively.When 15. 0% of enzymes and mycelia were removed daily,the total filter paper activity andβ-glucosidase activity increased by 42. 9% and 84. 3%,respectively,with mycelia kept at 4-5 g/L.Periodical enzyme recovery could reduce products inhibition or degrodation,and thereby to improve the cellulase productivity significantly when the fungal cell concentration is kept within a suitable range.
作者
王磊
王步成
勇强
余世袁
WANG Lei;WANG Bucheng;YONG Qiang;YU Shiyuan(Key Laboratory of Forest Genetics ＆ Biotechnology of the Ministry of Education,Nanjing Forestry University,Nanjing 210037,China)
出处
《生物加工过程》
CAS
2018年第4期72-79,共8页
Chinese Journal of Bioprocess Engineering
基金
"十二五"国家科技支撑计划(2015BAD15B09)
