摘要
研究了里氏木霉β-聚糖酶的生物合成,并比较了以玉米芯、纸浆、粗木聚糖为碳源对里氏木霉β-聚糖酶诱导合成的影响。结果表明:里氏木霉以15 g/L的玉米芯为碳源合成β-聚糖酶,培养4 d时滤纸酶活、羧基纤维素(CMC)酶活、纤维二糖酶活和木聚糖酶活分别为1.03 FPIU/mL、0.54、0.08和149.7 IU/mL;以纸浆为碳源,可得到较高纤维素酶活、较低木聚糖酶活的β-聚糖酶;以粗木聚糖为碳源,可制备低纤维素酶活的木聚糖酶,木聚糖酶活与CMC酶活的比值高达785.4,适合于纸浆的生物漂白。
The effects of carbon sources on the preparation of β-glycanases were investigated. The result showed： The filter paper activity,CMCase activity, cellobiase activity and xylanase activity were 1.03 FPIU/mL,0.54 IU/mL,0. 08 IU/mL and 149.7 IU/mL,respectively, when 15 g/L corncob employed as carbon source by Trichoderma reesei. The poor-xylanase cellulase was synthesized under 15 g/L kraft pulp as carbon source. The poor-cellulase xyla- nase,which was suitable for biobleaching,was formatted when the T. reesei cultivated in the same concentration of unpurified xylan,and the ratio of xylanase to CMCase was 785.4.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第6期88-90,共3页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目(30471361)
江苏省高技术研究计划(BG2005327)
