摘要
以纤维素酶水解蒸汽爆破麦秆的过程为研究对象,考察了底物浓度、纤维素酶用量、β-葡萄糖苷酶装载量以及化学激活剂对麦秆水解的影响。结果表明,高底物浓度下的最佳酶解工艺条件为底物（麦秆）浓度20%,酶装载量（U/g纤维素）：滤纸酶活45、β-葡萄糖苷酶25、木聚糖酶800,0.1 mmol/L Mg^2＋、0.1 mmol/L Co^2＋、10 mmol/L Fe^3＋,1 g/L PEG2000、1 g/L Tween80和1 g/L山梨醇,搅拌速度120-150 r/min,分批补料,pH4.8,50℃,水解时间144 h。在此条件下,还原糖浓度达115.43 g/L,葡萄糖浓度达88.39 g/L,转化率也分别达到78.04%和88.73%。
The cellulase hydrolysis process of steam explosion of straw as the research object was studied, the substrate concentration, dosage of cellulase, beta glycosidase enzymes loading capacity and the influence of chemical activator on straw hydrolysis. Results showed that high concentration of substrate optimum enzyme hydrolysis conditions for the substrate （straw） concentration 20%, enzyme loading capacity （U/g cellulase） ： 45 filter paper enzyme activity, 25 β-Glucosidases activity, 800 xylanase activity, 0.1 mmol/L Mg^2＋, 0.1 mmol/L Co^2＋, 10 mmol/L Fe^3＋, 1 g/L PEG2000, 1 g/L Tween80 and 1 g/L sorbitol, the agitation speed 1204150 r/min, and fed batch cultivation at pH4.8, 50℃ for 144 h. On this condition, the concentrations of the reducing sugar and glucose were achieved 115.43 g/L and 88.39 g/L respectively, the conversations were 78.04% and 88.73% respectively.
出处
《纤维素科学与技术》
CAS
CSCD
2015年第2期8-12,49,共6页
Journal of Cellulose Science and Technology
关键词
气爆麦秆
纤维素酶
高效水解
gas detonation straw
cellulase
efficient hydrolysis
