摘要
采用了DCC法制备了呕吐毒素完全抗原,通过LC/MS和ELISA法监控制备过程,结果显示,将呕吐毒素衍生化后,在4℃偶联反应20h,呕吐毒素与牛血清白蛋白偶联成功。呕吐毒素与牛血清白蛋白的初始摩尔比为50∶1,得到呕吐毒素完全抗原中呕吐毒素与牛血清白蛋白的摩尔比为9.7∶1。
DON artificial antigen was prepared with DCC Method in this paper.Liquid chromatography-Mass spectrometry （LC/MS） and ELISA were used to monitor the process for controlling the reaction during the preparation.The optimal conditions for preparation were established in this study.The DON artificial antigen was obtained with the molar ratio of 9.7：1 when thesubstances were derivatized and coupled for 20 h as the initial molar ratio of DON to BSA was 50：1.
出处
《食品研究与开发》
CAS
北大核心
2008年第5期118-121,共4页
Food Research and Development
