摘要
本研究采用间接竞争酶联免疫吸附试验(ELISA)检测黄曲霉毒素B1(aflatoxins B1,AFB1)残留量。将AFB1与蛋白质载体牛血清蛋白(BSA)和卵清白蛋白(OVA)偶联制备了AFB1-BSA和AFB1-OVA偶联物,以AFB1-BSA作为免疫原制备特异性抗体,并成功建立了AFB1残留间接竞争ELISA检测方法及检测试剂盒。试剂盒IC50为128.8ng/L,检测线性范围为50～1800ng/L,检测限不超过100ng/kg;食用油、花生和谷物的平均添加回收率大于71.3%,批内、批间变异系数均小于14.2%。因此,本试剂盒具有操作简便、检测快速、灵敏度高等特点,将在植物性食品中AFB1的残留检测中发挥重要作用。
This study was to aim to use ELISA method for rapid screening of the aflatoxins B1（AFB1）residues.Coupled AFB1to bull serum albumin（BSA）and ovalbumin（OVA）to obtain AFB1-BSA and AFB1-OVA respectively.Specific antibody against AFB1was prepared with AFB1-BSA and ELISA kit,and had been successfully developed for detecting AFB1residues.The IC50of the kit was 128.8ng/L,linear detection range was 50～1800ng/L with the lowest detection limit less than 100ng/kg.The recovery of AFB1in edible oil,peanut and cereal was more than 71.3%.Coefficient of variation for repeatability tests were less than 14.2%.Therefore,the kit was simple,fast,sensitive,and would be play an important role in AFB1residues detection in the plant food.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第1期57-61,共5页
China Animal Husbandry & Veterinary Medicine
