摘要
背景：目前较为常用的预防椎板切除术后硬膜外瘢痕粘连的措施是以各种半流体物质或者膜性材料作为硬膜与瘢痕之间的物理屏障，但效果并不令人满意。抑制作为修复细胞主要成分的成纤维细胞增殖，调节胶原代谢成为当前生物学防治病理性瘢痕的热点。目的：采用定性和定量结合的方法，比较局部应用不同浓度丝裂霉素C预防椎板切除术后硬膜外粘连的效果。设计：完全随机分组，对照动物实验。单位：连云港市第二人民医院骨科材料：选用健康成年SD大鼠40只，雄性，体质量200～220g，由南京医科大学动物实验中心提供。实验用药丝裂霉素C由日本协和发酵工业株式会社富士工厂生产，批号：425ACI，进口药品注册证号：H20020163。方法：实验于2004—06／10在南京医科大学动物实验中心及江苏大学医学院基础实验中心完成。随机将40只大鼠分为4组：丝裂霉素C高、中、低浓度组和生理盐水组，每组10只。将鼠麻醉后，切除LI椎板，形成0．2cm×0．5cm缺损，暴露硬脊膜。对照组以浸透生理盐水棉片，丝裂霉素C高、中、低质量浓度组以浸透质量浓度分别为0．1,0．05,0．01g／L的丝裂霉素C棉片置于裸露的硬脊膜后方5min。实验过程中对动物的处置符合动物伦理学标准。主要观察指标：手术后4周将每组麻醉后各处死4只动物，切取手术段脊柱硬脊膜外瘢痕组织，计算其中羟脯氨酸含量。其余动物在麻醉后，切取手术段脊柱包括其后侧瘢痕组织及骶棘肌，测不规则形态的瘢痕横切面面积，并计数纤维细胞数。光镜下评估各组标本硬膜外瘢痕粘连的程度。结果：大鼠40只均进入结果分析。对照组标本硬膜外瘢痕组织致密，与硬脊膜形成紧密粘连。丝裂霉素C高质量浓度组组无明显硬膜外粘连，瘢痕组织中羟脯氨酸含量、胶原组织面积及成纤维细胞计数
BACKGROUND： At present, using membrane materials to separate spinal dura mater and scar tissue or using semiliquid materials to surround the dimension of operation are very common after laminectomy. However, therapeutic effects are not satisfactory. Inhibition to the proliferation of fibroblasts, which are the main components of repairing cells, and regulating collagen metabolism have become a hot spot for preventing and heating pathological scar by a biological method.
OBJECTIVE： To compare the therapeutic effects of topical application of different concentrations of mitomycin C （MMC） on preventing peridural adhesion in rats after laminectomy by a qualitative and quantitative combined method.
DESIGN： A completely randomized grouping, controlled animal experiment.
SETTING： Department of Orthopaedics, Second People＇s Hospital of Lianyungang City.
MATERIALS： Forty healthy adult male SD rats, weighing 200-220 g, were provided by the Animal Experimental Center, Nanjing Medical University. MMC was produced in Union Fermentation Co.,Ltd （Batch No. 425ACI, Import drugs registration No. H20020163, Japan）.
METHODS： The study was performed at the Animal Laboratory Center, Nanjing Medical University, and the Basic Experimental Center, Medical College, Jiangsu University from June 2004 to October 2006. The 40 SD rats were randomly divided into 4 groups with 10 rats in each groulx MMC high-, medium-, and low-concentration groups, and a control group. After anesthesia, each rat was removal of Ll vertebral plate. A defect （0.2 cm × 0.5 cm） was created to expose dural mater. After hemostasia, cotton pads soaked either with various concentrations of MMC （0.1, 0.05, 0.01 g/L） or saline were covered on the laminectomy defects in the three MMC-treated groups and the control group for 5 minutes respectively. The protocol was carded out in accordance with ethical guidelines for the use and care of animals.
MAIN OUTCOME MEASURES： After the operation, 4 rats were randomly selected from all an
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第11期2185-2188,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
