摘要
[目的]比较局部应用不同浓度丝裂霉素C(MMC)预防椎板切除术后硬膜外黏连的效果。[方法]40只SD大鼠随机分为4组,分别切除L1椎板。术中各组分别以棉片浸透生理盐水(C组)、0.01 mg/m l(L组)、0.05mg/m l(M组)、0.1 mg/m l(H组)MMC,置于裸露的硬脊膜后5 m in。术后第4周处死动物取材,分别作肉眼观察、羟脯氨酸(HOP)含量测定、胶原组织面积测定及成纤维细胞记数。[结果]C组标本硬膜外瘢痕组织致密,与硬脊膜形成紧密黏连。H组无明显硬膜外黏连,HOP含量、胶原组织面积及成纤维细胞记数显著减少。M、L组HOP含量、胶原组织面积及成纤维细胞记数不同程度的减少,但不能避免硬膜外黏连的形成。[结论]局部应用浓度为0.1mg/m l的MMC能够有效减少硬膜外瘢痕组织增生,避免椎板切除术后硬膜外瘢痕黏连。
[ Objective] To compare the effects of topical different concentration mitomycin C （MMC） in preventing postlaminectomy peridural adhesion. [ Method ] Laminectomies were performed at L1 in 40 rats. Cotton pads soaked either with 0.01 mg / ml （group L）, 0.05 mg/ml （group M）, 0. 1 mg / ml （group H） MMC or saline （group C） were applied to the operative sites. The rats were killed 4 weeks after surgery. The specimens were prepared for determination of the degree of scar adhesion according to Rydell method, the content of Hydroxyproline （HOP）, the area of peridural fibrosis and the count of fibroblasts. [ Result] Dense peridural fibrosis with marked peridural adhesion showed in group C. No obvious adhesion formed in group H. In group M and L, peridural adhesion wasn＇t avoided. The content of HOP, the area of peridural fibrosis and the count of fibroblasts showed various degrees decrease in all MMC-treated groups. [ Conclusion] Local application of 0. 1 mg/ml MMC may be a successful method of reducing postlaminectomy peridural fibrosis and completely avoided peridural adhesion.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2007年第9期691-693,共3页
Orthopedic Journal of China
基金
连云港市卫生局资助项目(NO.05056)
