摘要
目的分析戊型肝炎病毒（HEV）各生物标志物之间关系以及各标志物在早期诊断中的作用。方法从临床收集急性散发性肝炎患者的血清，用酶联免疫（EIA）试剂检测HEV IgG抗体、IgM抗体和抗原，用实时荧光RT-PCR方法检测核酸，比较其相关关系。结果在121例急性散发性肝炎中，HEV IgM抗体阴性者为51例，其中抗原或／和核酸阳性者占45．1％；IgM抗体阳性者70份，其中抗原或／和核酸阳性者占67．1％。HEV IgG抗体在IgM抗体、抗原和核酸均阴性的患者中阳性率为53．6％；在IgM阴性而抗原或／和核酸阳性者中阳性率为73．9％；在IgM阳性的HEV感染者中阳性率为92．9％。HEV抗原与核酸的符合率为81．8％，抗原与IgM抗体的符合率为58．7％，而IgM抗体与核酸的符合率为53．7％。结论在HEV感染的早期诊断中增加抗原和核酸的检测有助于提高检出率。
Objective To analyze the relat-ionship among hepatitis E virus （HEV） biomarkers and their roles in early diagnosis of HEV infection. Methods One hundred and twenty-one sera were collected from patients with clinical diagnosis of sporadic acute hepatitis. All the sera were tested for anti-HEV IgG, IgM antibodies and antigen with enzyme immunology （EIA） and RNA with real-time fluorescent RT-PCR. Results Fifty-one of 121 sera were negative for anti-HEV IgM while 45.1% of them were positive for HEV antigen or/and RNA. The remaining 70 sera were positive for anti-HEV IgM while 67.1% of them were positive for HEV antigen or/and RNA. The positive rate of anti-HEV IgG in sera negative for anti-HEV IgM, antigen and RNA, in those negative for anti-HEV IgM and positive for antigen or/and RNA, in those positive for IgM was 53.6%, 73.9% and 92.9%, respectively. The concordance between HEV antigen and RNA, antigen and anti - HEV IgM , IgM and RNA was 81.8% , 58.7% and 53.7% , respectively. Conclusion Detection of HEV RNA and antigen besides detecting anti-HEV IgM would facilitate in early diagnosis of HEV infection.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2008年第1期6-9,共4页
Chinese Journal of Microbiology and Immunology
基金
国家863课题基金资助（2006AA02Z453）
