摘要
目的分析戊型肝炎病毒(HEV)IgM抗体阳性患者的入院时、住院中和出院时的系列血清中3种生物标志物的变化情况及其诊断意义。方法收集临床常规检测的HEV IgM抗体阳性患者的入院时、住院中和出院时的血清,用酶联免疫(EIA)试剂检测HEV IgG抗体和IgM抗体,用实时荧光PCR方法检测核酸,分析其变化情况。结果在25例HEV IgM抗体阳性患者系列血清中,HEV IgM抗体为4例高值无明显变化、15例高值明显下降变化、1例明显升高变化、5例低值无明显变化,HEV IgG抗体为3例高值无明显变化、15例高值明显升高变化、4例高值下降后维持高值、1例低值转为阴性、2例始终阴性,HEV RNA为入院第1天22例(88%)阳性、入院后1周15例(62.5%)阳性、入院后2周1例(5.6%)阳性。结论戊型肝炎患者发病过程中,系列血清HEV IgG抗体和IgM抗体及HEV RNA联合检测有助于戊型肝炎的明确诊断。
Objective To analyze the changes of three biomarkers of serial sera in patients with hepatitis E virus IgM antibody （HEV-IgM） positive during hospitalization and it＇s diagnosis value. Methods The serial serum samples were collected from patients with HEV-IgM positive during hospitalization. Enzyme immunoassay （EIA） was employed to detect anti-HEV IgG and IgM antibodies, and real-time fluorescent RTPCR was adopted to detect RNAs. The test results were analyzed. Results In 25 acses of serial sera from the patients with HEV-IgM positive, 4 cases kept HEV-IgM high OD value and no changed, 15 cases were high OD value and obviously decreased, 1 case had significantly elevated OD value, 5 eases kept HEV-IgM low OD value and no changed. HEV-IgG：3 cases kept HEV-IgG high OD value and no changed, 15 cases were high OD value and obviously changed, 4 cases were OD value significantly declined, 1 case was low OD value and transformed to negative, and 2 cases kept negative. The positive rate of HEV-RNA was 22/25（885） at day 1,15/24（62.5%） at wk 1,1/18（5.6%） at wk 2. Conclusion Combined detection of anti-HEV IgG, IgM and HEV RNA in serial sera during the course of disease contributes to diagnosis of hepatitis E.
出处
《国际检验医学杂志》
CAS
2009年第12期1158-1159,1162,共3页
International Journal of Laboratory Medicine
关键词
肝炎病毒
戊型
逆转录聚合酶链反应
免疫球蛋白M
免疫球蛋白G
Hepatitis E virus
Reverse transcriptase polymerase chain reaction
Immunoglobulin M
Immunoglobulin G
