摘要
为研究福建省2000～2003年急性散发性戊型肝炎病毒(HEV)分离株的分子流行病学特征,采用RT-PCR方法扩增HEVORF2的基因片段,经纯化、克隆、测序后,对其序列用Neighbor-Joining法和Bootstrap法进行基因进化分析.从158例急性散发性戊型肝炎的血清标本中扩增出72份HEV RNA,经基因进化树分析,这72株HEV均属于HEV基因Ⅳ型,且被明显地分为A、B、C、D共4个组群.A群的同源性最高,群内同源性为93.3%～100%;D群的同源性最低,群内同源性在86.6%～100%之间.A群和B群流行株所占比例没有差异;C群流行株所占比例由2002年的7.1%逐渐增加到2003年的26.3%(x2=10.553,P=0.014);D群则由85.7%逐渐减少到44.7%(x2=8.136,P=0.043).引起福建地区急性散发性戊型肝炎流行的HEV均属于HEV基因Ⅳ型,基因型内存在不同的组群,其中D群的变异较大.
To study the molecular epidemiological characteristics of hepatitics E virus isolates from year 2000-2003 in Fujian Province, nested RT-PCR was carried out to amplify the open reading frame 2（ORF-2） fragment of HEV gene of these isolates. The purified PCR products were cloned and then sequenced. Phylogenetic analysis was performed by Neighbor-Joining and Bootstrap. HEV RNAs were amplified from 72 sera of 158 serum samples from patients with acute sporadic hepatitis E, and all of which were characterized as HEV genotype Ⅳ, and could be divided into four distinct groups （A,B,C and D） . The sequence homology at the nucleotide level was in the range of 93.3% - 100% within group A, which was the highest among the four groups, while group D had the lowest sequence homology of 86.6% - 100%. During the four years, the proportions of groups A and B of HEV isolates did not significantly change, but that of group C gradually increased from 7.1% to 26.3 % （x^2 = 10.553, P = 0.014）, while that of group D gradually reduced from 85.7% to 44.7% （x^2 = 8. 136, P 0.043）. HEV isolates causing acute sporadic hepatitis E in Fujian Province belong to genotype Ⅳ. There have different groups within genotype Ⅳ, in which group D is with highest variability and unstability.
出处
《病毒学报》
CAS
CSCD
北大核心
2005年第5期353-357,共5页
Chinese Journal of Virology
基金
福建省科技重大专项(2004YZ01-32)
福州市科技发展基金资助项目(2002-21)
