摘要
目的 探讨抗 HEV阴性急性戊型肝炎病人的HEV基因型。方法 应用HEVORF2简并引物进行RT nPCR ,检测 2 2例抗 HEV阴性的急性肝炎病人血清HEVRNA ,应用双脱氧链终止法对部分阳性产物直接测序。结果 2 2例抗 HEV阴性的急性肝炎病人中 ,9例 (40 9% )HEVRNA阳性。对其中 6例阳性产物进行直接测序显示 ,5例为典型中国株 ,与HEV中国株核苷酸和氨基酸序列的同源性分别为98 0 %和 99 0 % ;1例变异较大 ,与HEV中国株、缅甸株、墨西哥株、美国株及猪HEV核苷酸序列的同源性分别为 77 4%、78 1%、74 3%、77 4%、77 1% ,氨基酸序列的同源性分别为 91 0 %、91 0 %、89 0 %、93 0 %、93 0 %。结论 我国除存在典型的中国株HEV感染外 ,还存在HEV变异株感染。
Objective To investigate genotypes of hepatitis E viruses isolated from hepatitis E patients with negative anti HEV. Methods Acute phase sera of 22 hepatitis patients with negative anti HAV IgM, HBsAg , anti HBc IgM, anti HCV and anti HEV were detected for HEV RNA using a RT nPCR based on a set of degenerate primers derived from open reading frame 2 of HEV. The positive PCR products were directly sequenced. Results Among 22 acute hepatitis patients, 9(40.9%) were HEV RNA positive. Six PCR products of 9 sera were directly sequenced. Five of them shared the same subtype with HEV Chinese strain (L25595), with the nucleotide identity of 98.0% and the amino acid identity of 99.0%. The remainder, designated HEV LZ 105, was a variant of HEV. The nucleotide and amino acid homology of HEV LZ 105 with HEV(China),HEV(Burma),HEV(Mexico),and the newly identified HEV US 1 and swine HEV was 77.4%,78.1%,74.3%,77.4%, 77.1% and 91.0%,91.0%,89.0%,93.0%,93.0%,respectively. Conclusion A variant of HEV was isolated from a hepatitis E patient with negative anti HEV.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2000年第2期164-166,共3页
Chinese Journal of Microbiology and Immunology
基金
"九五"国家科技攻关计划资助项目!(969060307)
