摘要
以鸡冠花顶芽为外植体,研究其离体快繁程序并在无菌体繁殖条件下探索了秋水仙素溶液不同浓度和时间处理对鸡冠花的诱变效应。快繁研究结果显示:最适初代培养基为MS+6-BA1.0mg/L+NAA0.2mg/L;以芽和茎段为培养对象诱导产生不定芽的最适培养基分别是MS+6-BA1.0mg/L+NAA0.1mg/L,MS+6-BA1.0mg/L+NAA0.02mg/L;最佳生根培养基是1/2MS+IBA0.4mg/L。采用浸泡法将鸡冠花营养芽在0.05%、0.1%、0.15%和0.2%不同秋水仙溶液浓度下分别处理12h、24h、36h和48h,并对诱导处理后获得的再生植株进行鉴定,得出以0.15%秋水仙素溶液浸泡36h为诱导多倍体的最佳处理组合,诱导率达23.3%。
The apical buds of Celosia cristata were used as the explants for the rapid propagation to compare the mutagenic effects treated with colchicines at different concentrations and time duration in tissue culture. The results of the rapid propagation showed that MS＋6-BA 1.0 mg/L ＋NAA 0.2 mg/L would be the optimum medium for generation; MS＋6-BA 1.0 mg/L ＋NAA 0.1 mg/L and MS＋6-BA 1.0 mg/L ＋NAA 0.02 mg/L might be the favorable proliferate medium for adventitious buds obtained from buds and stems; the best rooting medium would be 1/2 MS＋ IBA 0.4 mg/L. the apical bud of Celosia cristata was immersed in 0.05%, 0.1%, 0.15% and 0.2% colchicine solutions for 12 h, 24 h, 36 h, 48 h respectively and the chromosome numbers of the root tip cells in the regenerated plantlets were examined, which showed that the treatment with 0.15% colchicine solution for 36 h would be the best combination that the polyploid rate could reached 23.3%.
出处
《分子植物育种》
CAS
CSCD
2008年第1期187-192,共6页
Molecular Plant Breeding
关键词
鸡冠花
离体培养
多倍体
秋水仙素诱导
Celosia cristata, in vitro culture, Polyploid, Induced by colchicine
