摘要
采用浸泡和混培2种方法对萱草的愈伤组织和不定芽进行多倍体诱导探索。结果表明,2种试材在2种诱导方法下均能够获得多倍体;以愈伤组织作为外植体时,多倍体的诱导以氟乐灵浓度0.007%混培处理5 d的效果最好,成活率可达50%,诱导率达到35.74%;以不定芽作为外植体时,萱草多倍体的诱导以氟乐灵浓度0.01%浸泡处理6 h的诱导率最高,可达21.75%,成活率为26.67%。氟乐灵可以取代秋水仙素作为萱草多倍体诱导的有效试剂。
The callus and adventitious buds of Hemerocallis fulva were used to induce polyploid by soaking and mixed culture.The results showed that polyploid could be obtained by soaking method and mixed culture method,whether callus or adventitious bud.If callus was used as explant,the best effect of polyploid induction was treated with 0.007%trifluridine for mixed culture 5 d,the survival rate was 50%and the induction rate was 35.74%.If adventitious buds were used as explants,the highest induction rate of polyploid of Hemerocallis fulva could reach 21.75%and the survival rate was 26.67%,when the polyploid of Hemerocallis fulva was induced by 0.01%trifluridine for soaking 6 h.Therefore,trifluridine can replace colchicine as an effective reagent for polyploid induction of Hemerocallis fulva.
作者
李永平
李丽
梁峥
贾民隆
曹冬梅
LI Yongping;LI Li;LIANG Zheng;JIA Minlong;CAO Dongmei(Institute of Horticulture,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
出处
《山西农业科学》
2018年第12期1997-2000,共4页
Journal of Shanxi Agricultural Sciences
基金
山西省农业科学院攻关项目(YGG1604)
山西省科技成果转化引导专项二批(201604D132045)
关键词
萱草
氟乐灵
多倍体
Hemerocallis fulva
trifluridine
polyploid
