摘要
To confirm the inactivating effect of chito-oligosaccharides on Tobacco mosaic virus(TMV) particles in vitro,the difference of TMV pathogenicity was evaluated according to the decrease of local lesion numbers after inoculating with TMV mixed with chito-oligosaccharides(DP3-10)in Nicotiana glutinosa,and the virion structural change was studied by transmission electron microscopy after mixed with chito-oligosaccharides.In the range of tested concentrations of chito-oligosaccharides(100-1000μg /mL),the numbers of local lesions were strongly reduced with over 30% decrement,and the 88.4% reduction gained at the concentration of 600g /mL.It revealed that treatment with chito-oligosaccharide solution of 300-500μg /mL directly broke TMV particles into tiny pieces of 50-150nm long,and that treatment with solutions of 600-1000μg /mL caused virus particle agglomerated.The data presented here suggested that chito-oligosaccharides exerted strong inactivating effect on plant virus in vitro.
To confirm the inactivating effect of chito-oligosaccharides on Tobacco mosaic virus （TMV） particles in vitro, the difference of TMV pathogenicity was evaluated according to the decrease of local lesion numbers after inoculating with TMV mixed with chito-oligosaccharides（DP3-10）in Nicotiana glutinosa, and the virion structural change was studied by transmission electron microscopy after mixed with chito-oligosaccharides. In the range of tested concentrations of chito-oligosaccharides （100-1000μg /mL） , the numbers of local lesions were strongly reduced with over 30% decrement, and the 88.4% reduction gained at the concentration of 600g /mL. It revealed that treatment with chito-oligosaccharide solution of 300- 500μg/mL directly broke TMV particles into tiny pieces of 50-150nm long, and that treatment with solutions of 600-1000μg/mL caused virus particle agglomerated . The data presented here suggested that chito-oligosaccharides exerted strong inactivating effect on plant virus in vitro.
出处
《病毒学报》
CAS
CSCD
北大核心
2008年第1期76-78,共3页
Chinese Journal of Virology
基金
教育部长江学者和创新团队发展计划(No·200558)
西北农林科技大学博士启动基金
关键词
壳寡糖
烟草花叶病毒
钝化作用
chito-oligosaccharides
TMV
inactivating activity
