摘要
研究了 0 .3 %壳寡糖诱导番茄 (Lycopersiconesculentum )叶 12 0h后 ,其挥发性物质对番茄枯萎病菌 (Fusariumoxysporum )孢子萌发和菌丝生长的影响 ;采用气相色谱 质谱联用技术 ,检测诱导后番茄叶挥发性物质及植保素日齐素质和量的变化。结果表明 :经壳寡糖诱导后 ,番茄叶挥发性物质对病菌的抑制率较对照组的提高。番茄叶挥发性抗真菌物质总含量为对照组的 1.49倍 ;氧合脂类、萜类及芳香类化合物的含量分别提高了 61% ,10 %和 69%。其中 (E) 2 乙烯醛含量增加了 64 % ,水杨酸甲酯含量增加了 3 8% ,壳寡糖不能诱导番茄植保素日齐素的合成。
In the defensive responses of plants towards external pathogens, volatiles may play a potential role in plant chemical defence strategies. In our work, the leaves of Lycopersicon esculentum were treated with 0.3 % oligochitosan elicitor for 120h. The antipathogenic efficacy of volatiles after oligochitosan treatment was studied by using the method of inverted culture of conidial suspensions. The volatiles and phytolaexin rishitin in the leaves were analyzed by GC/MS. The results demonstrated that the inhibitory effect of volatiles against Fusarium oxysporum was enhanced in oligochitosan-treated leaves. Oligochitosan treatment also increased the amount of total antipathogenic volatiles, which was 1.49 times as compared with that of the control. Phyto-xylipins, terpenoids and phenols were increased by 61%, 10% and 69%, respectively. And there were 1.64-fold and 1.38-fold increases in (E)-2-Hexenal and Methyl Salicylate, respectively. In this system, rishitin was not detectable in oligochitosan-treated leaves.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第6期1008-1012,共5页
Periodical of Ocean University of China
基金
国家高技术研究发展计划项目 (2 0 0 2AA2 4 4 0 31 )资助
关键词
壳寡糖
诱导
番茄叶
挥发性抗真菌物质
日齐素
气相色谱-质谱联用
oligochitosan
induction
Lycopersicon esculentum leaves
Fusarium oxysporum antipathogenic volatiles
rishitin
GC/MS
