摘要
采用实时荧光定量PCR方法，研究了壳寡糖诱导对烟草体内TMV—CP基因表达量的影响。结果表明，壳寡糖诱导后烟草表达了对TMV侵染的系统获得抗病性，植株显症推迟4—7d，症状减轻，平均严重度比对照降低了82．9％。同时烟草叶片中cP基因的表达量显著降低。壳寡糖诱导处理的植株在接种病毒时（0h），CP基因的表达量（拷贝数／2μL）为0．918，接种后168h增加到167．730。而在不诱导对照植株内，CP基因的表达量在0h为1.218，接种后168h猛增到648．623。换言之，不处理对照的表达量在此期间增长了532．53倍，而壳寡糖诱导处理植株中仅增长了182．71倍。试验证明壳寡糖诱导的系统获得抗病性强烈抑制了烟草体内TMV—CP基因的表达。
The inhibitory effect to TMV-CP gene expression in tobacco （Nicotiana tabacum） seedlings induced by chito-oligosaccharides was examined with real-time quantitative RT-PCR （QPCR）. The results showed that high level systemic acquired resistance （SAR） was expressed in tobacco plants treated with solution of chito-oligosaccharides at the concentration of 50μg/mL, the latent period was 4 - 7 d delayed and the average disease severity was 82.9% lowered. Meanwhile, the expression quantity （copies/2 μL） of CP gene in treated plants was reduced remarkably, it was 0. 918 at 0 h （the time of virus inoculation） and 167. 730 at 168 h after inoculation. But, in untreated plants the expression quantity was 1. 218 at 0 h, and 648. 623 at 168 h. During this time span that increased 532.53 times in untreated plants, and only 182.71 times in treated plants. It was concluded that the expression of TMV-CP gene in tobacco was strongly suppressed by SAR induced by chito-oligosaccharides.
出处
《植物病理学报》
CAS
CSCD
北大核心
2007年第6期637-641,共5页
Acta Phytopathologica Sinica
基金
教育部长江学者和创新团队发展计划资助项目(200558)
西北农林科技大学博士启动基金资助项目
关键词
壳寡糖
系统获得抗病性
TMV—CP基因
实时荧光定量PCR
chito-oligosaccharides
systemic acquired resistance （SAR）
TMV-CP gene
real-time quanti tative RT-PCR
