摘要
目的:单纯生物蛋白胶是一种良好的缓释载体,拟通过与转化生长因子β1抗体复合应用于肌腱损伤局部,观察其对鞘管区屈肌腱粘连的预防作用和对肌腱愈合的影响。方法:实验于2003-01/04在解放军第四军医大学西京医院骨科研究所完成。①实验动物:将72只来亨鸡(leghorn)按随机化原则分为单纯转化生长因子β1抗体组、单纯生物蛋白胶组、转化生长因子β1抗体复合生物蛋白胶组及生理盐水组,每组18只。实验过程中对动物处置符合动物伦理学要求。②实验方法:分别将鸡左足第三、四趾趾深屈肌腱横断,作The 4-strand Cruciate repair缝合,然后按照分组,用1 mL无菌注射器,向鞘管区分别注入0.2 mL转化生长因子β1抗体(8 mg/L)和生理盐水,用生物蛋白胶专用注射器向鞘管区分别注入0.2 mL生物蛋白胶和转化生长因子β1抗体生物蛋白胶复合液。③实验评估:术后5 d行早期功能锻炼,在第1,3,8周麻醉后处死取材,第四趾标本分别行大体观察、组织学检查和缝合处粘连评分。第三趾在SHIMADZU SPL-10kN型材料实验机上进行生物力学测试。结果:转化生长因子β1抗体复合生物蛋白胶组术后3,8周,缝合处粘连程度评分、肌腱滑动距离、模拟主动屈曲度及屈曲功均优于其余3组,且差异有显著性意义(P<0.05)。4个处理组的最大抗断裂载荷差异无显著性意义(P>0.05)。结论:转化生长因子β1抗体复合缓释载体生物蛋白胶可以有效预防术后肌腱粘连,并且不影响肌腱的正常愈合进程。
AIM： Fibrin glue is a kind of good slow-release carriers, we examined the effect of transforming growth factor （TGF）-β1 antibody and fibrin glue compound in preventing tendon adhesion in the area of tendon sheathing canal and healing injured tendon. METHODS： The experiment was carried out in the PLA Orthopedic Institute of the Fourth Military Medical University of Chinese PLA from January to April in 2003.（1）Totally 72 leghorn chickens were divided into four groups randomly, and 18 chickens were included in each group. Group 1 simply used TGF-β1 antibody. Group 2 used only fibrin glue, Group 3 used TGF-β1 antibody and fibrin glue compound, Group 4 used normal saline, All the disposals were in accordance with the ethical standard of animals.（2）The third and fourth flexor digitorum profundus tendons of the chicken were transverse broken and then sutured using the 4-strand cruciate repair method. Afterwards 0.2 mL TGF-β1 antibody （8 mg/L） and normal saline were injected into the area of tendon sheathing canal using 1 mL sterile syringe, 0.2 mL TGF-β1 antibody and fibrin glue compound was injected using special syringe, according to the above groupings. （3）Early functional exercise was carried out 5 days after operation. The animals were sacrificed during the 1, 3 and 8 weeks, respectively. The sample of the fourth flexor digitorum profundus tendon was observed in general and histologically. The adhesion at the sutured site was also scored. Biodynamic function of the third flexor digitorum profundus tendon was detected using Shimadzu Spl-10kn machine. RESULTS： The tendon adhesion score, tendon gliding distance, simulative active flexion and flexion function in group 3 were superior than that in the other 3 groups 3 and 8 weeks after operation. The difference was significant statistically （P 〈 0.05）. The maximum anti-broken load in the four groups had no difference statistically （P 〉 0.05）, CONCLUSION： TGF-β1 antibody and fibrin glue compound is helpful to prevent postoper
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第48期9637-9640,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
山东省卫生厅计划基金资助项目(2003CA1CFB1)~~
