摘要
通过生物测定和地高辛(DIG)标记的酶联免疫吸附剂测定(ELISA),初步分析了家蚕对浓核病毒中国(镇江)株(Bombyxmori densovirus,BmDNV-z)的抵抗性机制。试验表明:供试家蚕品种的消化液、血液中均不存在对BmDNV-z侵染性有影响的蛋白因子;抵抗性家蚕品种的中肠组织蛋白中不存在BmDNV-z的病毒受体蛋白,而感受性家蚕品种的中肠组织蛋白中可能存在对BmDNV-z侵染性有影响的蛋白因子,即感受性家蚕品种的中肠组织中可能存在BmDNV-z的病毒受体蛋白因子,且BmDNV-z与感受性家蚕品种中肠组织蛋白的结合具有组织特异性。
To investigate the mechanism of silkworm resistance to Bombyx moil densovirus （BmDNV-z）, the infectivity of virus to silkworm after incubated with extract of different tissues of silkworm of susceptible and non-susceptible varieties was tested by bio-assay, and the combination activity of BmDNV-z with proteins from the extracts was measured by DIG-labeled ELISA. The results indicated that there were no protein factors affecting the invasion of BmDNV-z in the digestive juice and the blood of the silkworm. Some protein factors which enhanced the invasion of BmDNV-z existed in the midgut of susceptible variety. We speculated that protein factor as virus receptor may exist in the midgut of susceptible variety, but not in the non-susceptible variety. The combination activity was tissue-specific to the midgut tissue of susceptible variety.
出处
《蚕业科学》
CAS
CSCD
北大核心
2007年第4期596-601,共6页
ACTA SERICOLOGICA SINICA
基金
国家重点基础研究发展计划"973"项目(编号2005CB-121004)
国家科技支撑计划项目(编号2006BAD06B03)
国家自然科学基金项目(编号30400327)
关键词
家蚕
中肠组织
抵抗性
浓核病毒
Bombyx mori
Midgut tissue
Resistance
Densovirus
