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洋葱中蒜氨酸酶的分离纯化 被引量:3

Separation and purification of Alliinase from onions
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摘要 研究以国产红皮洋葱为原料,通过硫酸铵分级沉淀、Sephacryl S-200HR柱层析、Con A-Sepharose4B亲和层析等处理,对洋葱中的蒜氨酸酶进行了分离和精提,得到纯化8.05倍、回收率49.5%的蒜氨酸酶,并通过聚丙烯酰胺凝胶电泳,测定洋葱中蒜氨酸酶亚基分子量为50.2ku。 To separate and purify Alliinase from Chinese red onions, we optimize saturated (NH4)2SO4 of 30%~65%, Sephacryl S-200HR and Con A-Sepharose 4B to get the purified onions Alliinase of 8.05, total recovery of 49.5% and subunit molecular weight of 50.2ku.
出处 《食品科技》 CAS 北大核心 2007年第11期84-86,共3页 Food Science and Technology
基金 国家自然科学基金项目(30671470)
关键词 洋葱 蒜氨酸酶 可溶性蛋白质 纯化 onions Alliinase soluble protein purification
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参考文献4

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二级参考文献32

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