摘要
目的:探讨CD147siRNA对恶性黑色素瘤成瘤及增殖活性和血管新生的影响。方法:建立pSUPER/CD147siRNA转染恶性黑色素瘤A375细胞皮下异种移植裸鼠模型并观察肿瘤生长情况;应用免疫组织化学方法检测裸鼠肿瘤组织内增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)和CD31的表达,依据PCNA的表达判断细胞的增殖活性,CD31的表达评价肿瘤微血管密度。结果:实验组成瘤率与对照组相比无统计学差异(P>0.05),但实验组肿瘤体积显著缩小(P<0.01)。实验组PCNA的表达和血管密度明显低于对照组(均P<0.01)。结论:CD147siRNA在裸鼠体内可显著抑制恶性黑色素瘤生长和血管新生,CD147分子可能是基因治疗恶性黑色素瘤一个新的靶分子。
Objective To investigate the effect of pSUPER/CD 147 siRNA proliferation, and angiogenesis of malignant melanoma in nude mice. Methods on the formation, The nude mouse subcutaneous xenotransplantation models of malignant melanoma were constructed and observed using morphological analysis. The protein expression of PCNA and CD31 in tumors was detected using immunohistochemical technique. Tumoral proliferative activity in the nude mice was quantitatively evaluated by proliferation cell nuclear antigen （PCNA）. Microvessel density （ MVD ） was counted based on the endothelial cells positively stained with anti-CD31 antibody. Results The subcutane- ous tumors appeared in all the nude mice 5 days later after the transplantation, but the volume of ma- lignant melanoma in the experiment group significantly decreased compared with the control group （P 〈 0.01 ）. PCNA expression and MVD were significantly lower in the experiment group than those in the control group （ P 〈 0.01 ）. Conclusion CD147 siRNA inhibits the growth and angiogenesis of malignant melanoma in vivo, suggesting that CD147 might be a new gene therapy target molecule for malignant melanoma.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2007年第5期791-795,共5页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(30571682)
湖南省杰出青年基金(06CB001)
湘雅医院"百万人才战略工程"基金~~
