摘要
目的:研究CD147 siRNA转染对肺腺癌细胞系A2中CD147表达及细胞增殖的影响,探讨CD147 siRNA转染对肺腺癌细胞系的作用。方法:用脂质体LipofectamineTM2000介导CD147 siRNA转染人肺腺癌细胞株A2,用RT-PCR检测CD147 mRNA表达,并用MTT法检测CD147 siRNA转染对A2细胞增殖的影响。结果:转染后的肺腺癌细胞株A2中CD147 mRNA表达水平明显下调,转染了CD147 siRNA的细胞在24、48和72 h的增殖能力较A2分别下降了48.3%、51.8%和53.2%(P均<0.05)。结论:CD147 siRNA转染能有效降低肺腺癌细胞株A2中CD147的表达,且能抑制肿瘤细胞的增殖能力。CD147 RNA干扰有可能成为肺腺癌基因治疗的新途径。
OBJECTIVE： To investigate the expression of CD147 siRNA on the expression of CD147 and the proliferation of lung adenoeareinoma cell line A2. and study the biolgical effects of targeting CD147 RNA interference on lung adenocarcinoma cell line. METHODS： CD147 siRNA was transfected into lung adenocarcinoma cell line A2 by LipofectaminerM2000, CD147 expres sion was monitored by RT PCR, and the proliferation of the cells by MTT assay. RESULTS： After the interference of CD147 siRNA, the mRNA expression of CD147 in the A2 cells was significantly down-regulated. The proliferation levels of transfected A2 cells at 24, 48 and 72 h after the interference decreased significantly by 48. 3%,51. 8% and 53. 2% （P〈0.05）. CONCLUSIONS： CD147 siRNA can effectively inhibit the expression of CD147 in A2 cells, and inhibit the proliferation of the cells. It is demonstrated that targeting CD147 RNA interference can potentially be a new approach for lung adenoearcinama gene therapy.
出处
《中华肿瘤防治杂志》
CAS
2011年第11期836-839,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
辽宁省教育厅高等学校科研基金(2008760)
辽宁省自然科学基金(2009225051-3)
关键词
肺肿瘤
CD147
腺癌
细胞增殖
lung neoplasms
CD147
adenocarcinoma
cell proliferation
