摘要
目的观察大鼠骨髓间充质干细胞(MSCs)与血管平滑肌细胞直接接触培养后向血管成分细胞的分化及超微结构改变。方法将MSCs(DAPI标记)与血管平滑肌细胞按一定的比例混合培养后,用平滑肌细胞抗体SM-α-actin、calponin免疫荧光染色,并用透射电镜观察MSCs的超微结构改变,检测MSCs的分化情况。结果MSCs与血管平滑肌细胞混合培养5d后免疫荧光染色,可见胞核蓝染的MSCs与抗SM-α-actin、抗calponin(绿色)的双标细胞出现;而单独培养的MSCs抗SM-α-actin阳性,抗calponin为阴性。并且混合培养后的MSCs电镜下可见肌丝状结构。结论血管平滑肌细胞直接接触可以诱导MSCs向血管平滑肌细胞分化。
Objective To study the effect of direct contact with vascular smooth muscle cells on transdifferentiation and ultrastructure of mesenchymal stem cells（MSCs）. Methods Rat MSCs and vascular smooth muscle cells were cultured and identified, respectively. MSCs were labeled with DAPI firstly, and then co-cultivated with vascular smooth muscle cells for 5d. MSCs were cultured alone as a control. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against smooth muscle α-actin and calponin, Results The MSCs cocultured with vascular smooth muscle cells expressed smooth muscle α-actin and calponin at 5d of cultivation,no cells positive for calponin were detected in the control group;and a lot of filaments were observed in the co-cultured MSCs by electron microscopy. Conclusion Cell-to-cell contact can induce rat MSCs to differentiate into vascular smooth muscle-like cells.
出处
《重庆医学》
CAS
CSCD
2007年第13期1278-1279,1282,F0002,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(30400180)
关键词
骨髓间充质干细胞
血管平滑肌细胞
共培养
分化
mesenchymal stem cells
vascular smooth muscle cells
co-culture
differentiation
