摘要
目的探讨大鼠骨髓干细胞种植于豚鼠脱细胞血管支架方法构建组织工程血管的免疫原性。方法选取健康纯系雄性SD大鼠80只，体质量200～250g，随机分为4组，皮下包埋不同移植物：骨髓干细胞种植组（A组，种植大鼠骨髓干细胞的脱细胞豚鼠血管，n=20）、单纯脱细胞组（B组，单纯脱细胞的豚鼠血管，n=20）、新鲜异种血管组（C组，新鲜豚鼠血管，n=20）、假手术组（D组，不包埋任何标本，n=20）。术后第3、7、15、30、60天每组处死4只大鼠，获取大鼠血清并取出移植物。苏木素-伊红（HE）染色观察豚鼠血管脱细胞处理效果及移植物表面炎性反应强度，免疫组织化学方法检测移植物表面抗豚鼠血管IgG和补体C3沉积。结果（1）HE染色显示每组移植物中均可见明显炎性细胞浸润，A组及B组的炎症浸润程度明显低于C组。（2）免疫组织化学法检测：各组移植物表面有明显的抗豚鼠血管IgG沉积。术后3d各组平均吸光度（IA）值差异无统计学意义（A组0．1506±0．0394，B组0．1632±0．0340，C组0．1668±0．0510，P〉0．05）。术后7、15、30、60dC组平均似值（0．2157±0．0452、0．2563±0．0442、0．2752±0．0579、0．2545±0．0466）高于A组（0．1633±0．0388、0．1787±0．0532、0．1692±0．0406、0．1633±0．0282）、B组（0．1555±0．0348、0．1740±0．0364、0．1698±0．0330、0．1621±0．0349，P〈0．05）。术后各时间点A组较B组均差异无统计学意义（P〉0．05）。（3）免疫组织化学法检测显示移植物表面有明显的大鼠补体c3沉积。术后3、7、15d各组平均M值差异无统计学意义（A组0．1304±0．0313、0．1552±0．0425；B组0．1316±0．0254、0．1535±0．0396：C组0．1671±0．0422、0．1766±0．0493，P〉0．05）。术后30、60dC组平均M值（0．2401±0．0706、0．2674±0．0788、0．2044±0．0494）高于A组（0．1612±0．0358、0．1597±0�
Objective To explore the immunogenicity of tissue-engineering blood vessels construc- ted by acellular guinea pig＇ s arteries planted with rat＇ s mesenchymal stem cells （MSCs）. Methods Eighty healthy clonic masculine clean Sprague-Dawley （SD） rats weighing 200-250 g were selected, and randomly devided into 4 groups which were transplanted with different samples （ group A ： tissue-engineer- ing blood vessels planted with rat＇ s MSCs, n = 20 ; group B ： acellular guinea pig＇ s arteries, n = 20 ; group C ： fresh guinea pig＇ s arteries, n = 20 ; group D ： sham operation, n = 20）. Four rats of each group were ex- ecuted on the 3rd, 7th, 15th, 30th and 60th day after the operation, and serum and grafts of rats were ob- tained. The acellular arteries and inflammatory reaction on the grafts＇ surface were observed by hematoxy- lin and eosin （HE） staining. The concentrations of anti-guninea pig＇s arteries IgG and complement C3 in serum and their deposition on the grafts＇ surface were tested by using immunohistochemistry. Results （1） HE staining： There was obvious inflammatory cell infiltration in the grafts in all groups, and infihra-tion of inflammatory cells was significantly more severe in group C than that in groups A and B ; （2） Immu- nohistochemistry showed that there was much IgG deposited on the the grafts＇ surface. On the 3rd day, the average absorbance （A） in the grafts had no significant differences among groups A （0. 1506 ± 0. 0394） , B （0. 1632 ±0. 0340） and C （0. 1668 ± 0. 0510, P 〉 0.05）. The average A values at 7th, 15th, 30th and 60th day postopration in group C （0. 2157 ±0. 0452, 0. 2563 ±0. 0442,0. 2752 ±0. 0579,0. 2545 ± 0. 0466） were higher than those in groups A （0. 1633 ± 0. 0388, 0. 1787 ± 0. 0532, 0. 1692 ± 0. 0406, 0.1633±0.0282） and B （0.1555 ±0.0348, 0.1740±0.0364, 0.1698±0.0330, 0.1621 ±0.0349, P 〈 0. 05 ）. There were no significant differences between groups A and B （ P 〉 0. 05 ） ; （ 3 �
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第8期1631-1634,共4页
Chinese Journal of Experimental Surgery
基金
中国博士后基金资助项目（57068）
关键词
骨髓干细胞
组织工程血管
免疫原性
IGG
Bone marrow mesenehymal stem cell
Tissue engineering vessel
Immunogeniei- ty
IgG
