摘要
目的探讨淋球菌青霉素耐药性与青霉素结合蛋白1基因和2基因(ponA、penA)突变的关系。方法采用琼脂稀释法对淋球菌临床分离株进行青霉素敏感性测定,分别运用聚合酶链反应-单链构象多态性(PCR-SSCP)和PCR-限制性片段长度多态性(RFLP)对淋球菌penA及ponA基因进行分析。结果在检测出的80株淋球菌临床分离株中,所有菌株的penA基因均发生了(Asp- 345A)的插入突变,而菌株表现出对青霉素不同程度的耐药性;通过RFLP分析检测出有近93.7%的菌株发生了ponA基因第421位氨基酸由亮氨酸变成脯氨酸(Leu421→Pro)的突变。同时研究还表明所有的PPNG菌株也可同时发生penA基因的突变,除2例ponA基因未突变外,94.4%(34/36)的PPNG可发生ponA基因的突变。结论在淋球菌流行株中染色体介导和质粒介导两种方式协同作用造成了淋球菌对抗生素的高度耐药性。
Objective TO investigate the relationship between penA/ponA and penicillin resistance of Neisseria gonorrhoeae. Methods Agar dilution method was used to determine the minimum inhibitory concentrations （MICs） of the strains. Polymerase chain reaction-single stand conformation polymerphism （PCR-SSCP） and PCR- restriction fragment length polymorphism （RFLP） were used to detect the mutations in ponA and penA genes, which encoding the penicillin binding protein-1 and -2 （PBP1 and PBP2）, respectively. Results All the 80 N. gonorrhoeae isolates had a D345 insertion detected in penA while 93.7% of N.gonorrhoeae isolates having a point mutation Leu421→Pro in ponA. Most of the penicillinaseproducing N. gonorrhoeae （PPNG） strains possessed the mutations in ponA and penA. Conclusion Our data suggested that the plasmid and chromosome mediated penicillin-resistance conjugately increased the level of resistantce.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2007年第9期901-905,共5页
Chinese Journal of Epidemiology
关键词
淋球菌
青霉素
单链构象多态性
限制性片段长度多态性
Neisseria gonorrhoeae
Penicillin
Single stand conformation polymerphism
Restriction fragment length polymorphism
