摘要
目的:建立中成药金匮肾气丸(水蜜丸)中丹皮酚的含量测定方法,便于控制制剂质量.方法:采用高效液相色谱法(HPLC法),色谱柱Prodigy 5u ODS3 1000A(250mm×4.6mm),以甲醇—水(55:45)流动相,检测波长274nm,流速1.0ml/min。结果:丹皮酚在0.5784μg/ml～9.2544μg/ml浓度范围内与峰面积呈良好线性关系,r=0.9992,回收率99.61%。结论:该法简便快捷.结果准确,可用于金匮肾气丸(水蜜丸)中丹皮酚的含量测定和质量控制。
Objective： To establish HPLC; method for determination of paeonol in Jinkuishenqi Pills, and is suitable for the use of quality control of paeonol in Jinkuishenqi Pills.. Methods： HPLC analys is was carried at by using Prod igy 5u ODS3 100A（250mm × 4.6mm）column, the mobile phase was methanol-water（55：45）, detection wavelength was 274nm, And the flow rate of 1.0mL/min. Results： A good linearity between the range of 0. 5784μg/ml～9. 2544μg/ml was found（r = 0. 9992）, the average recovery was 99.61%. Conclusion： The method was simple, accurabe and is suitable for the use of quality control of paeonol in Jinkuishenqi Pills.
出处
《黑龙江医药》
CAS
2007年第5期427-428,共2页
Heilongjiang Medicine journal
