摘要
根据SRAP引物设计规律设计了一套引物,结合混合集群分析法(BSA),对油菜隐性细胞核雄性不育两用系430AB的不育与可育DNA池进行筛选,用在不育和可育DNA池间存在差异的引物再对430AB群体进行检测,获得了与隐性核不育基因m s连锁的3个SRAP标记,即m11 e33270、m16 e7280和m16 e17370,它们位于核不育基因的同一侧,遗传图距分别为4.7 cM、20.1 cM和26.4 cM。
A set of designed SRAP primers, combining with bulked segregation analysis （BSA） was used in a genic male sterile line 430AB of Brassica napus L, to identify the molecular markers linked to recessive genic male sterile gene. These SRAP primers which generated polymorphic bands between the DNA pools of male fertile and the male sterile plants were used to test the whole population of 135 individual plants of 430AB. Three SRAP markers, ml le33 270, m16e7 280and m16e17 370 , were identified to be linked to the recessive genic male sterile gene, They were located at the same side of the male sterile gene with genetic distances of 4. 7 cM, 20. 1 cM and 26.4 cM, respectively.
出处
《江苏农业学报》
CSCD
北大核心
2007年第4期283-288,共6页
Jiangsu Journal of Agricultural Sciences
基金
农业部"948"项目(2006Q004)
江苏省高技术项目(BG2004307
BG2005308)
江苏省农业科学院基金项目(6110405)
