摘要
以低芥酸油菜隐性胞核雄性不育材料１１７Ａ和双低不育材料３８８Ａ与黄籽双低品系杂交，Ｆ１去雄正反回交，以ＢＣ１后代Ｂ２代中的Ａ株与原父本国交，再与Ｂ３中的Ａ株测艾转育，经７代即可有效地育成黄籽频率在８０％以上、硫苷含量＜３０μｍｏｌ／ｇ、芥酸含量＜１％、且植株性状整齐而稳定的黄籽双低隐性核不育两型系。以Ｂ３Ａ×ＢＣ２测交一代的Ｂ株作选黄籽测硫苷选育，其黄籽频率虽可达到８７．７％～９１．６％，但需花费８代时间。以ＢＣ１后代Ｂ２或Ｂ３代Ｂ株选黄籽测硫苷选育，虽可在５或６代育成，但其黄籽频率只能保证在７０％左右，硫苷含量也都不很稳定。
A, a recessive genetic male sterile material with low erucic acid content and 388A, a canola sterile material were crossed to yellow canola lines. F1s were backcrossed reciprocally after emasculated. Again the A plants of B2 from BC1 were backcrossed to their orignal parents. then they were testcrossed to the A plants of B3. After 7 generations of transferring, ayellow seed canola recessive genetic sterile line with yellow frequency over 80%, glucosinolate content <30μmol/g and erucic acid content<1% could be developed. If the B plants from B3A × BC2 were used for selecting yellow seed and low glucosinolate, it would need 8 generations though the yellow seed rate might be 87. 7%～91. 6 %. If the B2 plants from BC1 or the Bplants of B3 were used for yellow seed and low glucosinolate selection, this proecedure might be finished in 5 or 6 generations, however, the yellow seed rate might be only about 70% and the glucosinolate contents would be unstable.
出处
《西南农业学报》
CSCD
1996年第3期33-37,共5页
Southwest China Journal of Agricultural Sciences
关键词
甘蓝型油菜
隐性核不育
黄籽双低转育
回交
测交
Brassica, napus
Recessive GMS
Yellow-seed canola transfering
Backcross Testcross
