摘要
通过对杉木(Cunninghamia lanceolata Hook)再生系统的比较研究,建立了子叶、下胚轴、茎段和针叶等四个再生体系。子叶在附含1mg/L BA和0.1mg/L NAA的DCR培养基中再生频率为93.7%±0.45%,平均芽数为3.76±0.25;下胚轴在附含2mg/L BA和0.2mg/L NAA的DCR培养基中再生频率为96.2%±0.35%,平均芽数为17.4±0.18;茎段在附含1mg/L BA和0.1mg/L NAA的DCR培养基中再生频率达100%,平均芽数为3.28±0.11;针叶在附含1.5mg/L BA和0.1mg/L NAA的DCR培养基中再生频率达84.5%±0.45%,平均芽数仅为1.42±0.08。不定芽在附含0.2mg/L BA和0.02mg/L NAA的DCR培养基中有效伸长。芽苗经预处理后在附含0.3mg/L IBA的1/2 DCR培养基中生根效果最佳。
Four regeneration systems derived from cotyledon, hypocotyls, stem and needle explants have been established in the comparative researches of regeneration systems in Cunninghamia lanceolata Hook. A high frequency （93.7%±0.45%） of adventitious buds were induced from cotyledons on DCR medium supplemented with 1 mg/L BA and 0.1mg/L NAA, with a maximum mean number buds of 3.76±0.25. A higher frequency （96.2%±0.35%） of adventitious buds were induced from hypocotyls on DCR medium supplemented with 2 mg/L BA and 0.2 mg/L NAA, with a maximum mean number buds of 17.4±0.18. A highest frequency （100%） of adventitious buds were induced from stem segments on DCR medium supplemented with 1 mg/L BA and 0.1mg/L NAA, with a maximum mean number buds of 3.28±0.11. A low frequency （84.5%±0.45%） of adventitious buds were induced from needles on DCR medium supplemented with 1.5 mg/L BA and 0.1mg/L NAA, with a maximum mean number buds of 1.42±0.08. Buds were elongated on DCR medium supplemented with 0.2 mg/L BA and 0.02 mg/L NAA. After pre-treating shoots, best rooting result was produced on 1/2 DCR medium supplemented with 0.3 mg/L IBA.
出处
《分子细胞生物学报》
CSCD
北大核心
2007年第4期239-244,共6页
Journal of Molecular Cell Biology
关键词
杉木
组织培养
器官发生
再生系统
遗传转化
离体快繁
Cunninghamia lanceolata. Tissue culture. Organogenesis. Regeneration system.Genetic transformation. In vitro propagation
