摘要
应用正交设计法研究外植体的最佳消毒方式,同时考察了培养基、6-BA、KT、NAA等4个因子对杉木组培苗芽增殖的影响和IBA、NAA、ABT1#、蔗糖等4个因子对组培苗生根培养的影响。结果表明:以茎尖为外植体材料,消毒方式采用70%乙醇处理40 s后无菌水冲洗一遍,再用0.1%升汞溶液处理7 min后用无菌水冲洗5次以上;最佳的增殖培养基是1/2MS+6-BA 0.8 mg·L-1,最适于生根的培养基是MS培养基中+蔗糖30 g.L-1+IBA 1.2 mg·L-1+NAA 0.2 mg·L-1+ABT1#1.5 mg·L-1。
The orthogonal design was used to select the optimal method for the explant disinfection. This paper studied the effects of 4 factors, medium, 6-BA, KT and NAA, on the bud propagation, and the effects of 4 factors, IBA, NAA, ABT1^# and sucrose, on rooting in tissue culture. The result showed that the stem tips should be used for explants; the disinfection method was using 70 % alcohol for 40 s then rinsed with autoclaved water once, followed by 0.1% HgCl2 for 7 min then rinsed with autoclaved water 5 times; 1/2MS medium supplemented with 6-BA 0.8 mg·L^-1 was suitable for its multiplication, and MS supplemented with sucrose 30 g·L^-1＋IBA 1.2 mg·L^-1＋NAA 0.2 mg·L^-1＋ABT1^#1.5 mg·L^-1.
出处
《福建林业科技》
北大核心
2007年第2期133-136,共4页
Journal of Fujian Forestry Science and Technology
关键词
杉木
无性繁殖
组织培养
正交试验
Chinese fir
clone propagation
tissue culture
orthogonal experiment
