摘要
以"黄天霸"百合的小球茎作为外植体,研究茎尖组培快繁技术体系,试验结果表明:外植体用6%NaClO溶液消毒10 min+0.1%HgCl2消毒10 min交替使用效果最佳,外植体存活率达72.0%;切取茎尖培养在MS+KT 0.4 mg/L+2,4-D1.0 mg/L培养基上,愈伤组织诱导率可达88.3%。组织培养中细胞分裂素和生长素之间的浓度比,可以有效控制植物组织培养过程中芽和根的发生。本研究中,不定芽诱导的最佳培养基配方为MS+KT 1.0 mg/L+IBA 0.5 mg/L,芽苗分化系数达3.44,生根培养的最佳培养基配方为:1/2 MS+IBA 0.4 mg/L+NAA 0.3 mg/L,生根率达96.7%。生根苗移栽后经过40 d的精心培育,成活率可达96%以上。
Using bulblet of Lilium orential ＂manissa＂ section as the explants,its propagation tissue culture techniques of stem tips were studied.The results revealed that survival rate of explants reached 72.0% by the surface sterilization with 6% NaClO solution for 10 minute and 0.1% HgCl2 solution for 10 minute;Cut stem tip cultured in the MS＋KT 0.4 mg/L＋2,4-D 1.0 mg/L,callus induction frequency can reach 88.3%.The concentration ratio between cytokinin and auxin in tissue culture can effectively control the process of buds and roots occurred on plant tissue culture.In this study,the best medium formula for adventitious bud induction was the MS＋ KT 1.0 mg/L＋IBA 0.5 mg/L,differentiation factor of bud seedling up to 3.44,and 1/2 MS＋IBA0.4 mg/L＋ NAA0.3 mg/L was suitable for its rooting,rooting rate reach 96.7%.The seedlings transplanted after 40 d of nurturing,survival rate reached above 96%.
出处
《江西林业科技》
2013年第2期13-17,共5页
Jiangxi Forestry Science and Technology
基金
福建省发改委农业"五新"推广科学基金资助项目(2008-31)
关键词
“黄天霸”百合
茎尖
愈伤组织
植株再生
Lilium orential ＂mantissa＂
stem tips
callus induction
plant regeneration
