摘要
目的:评估癌胚抗原(CEA)mRNA在诊断胃癌细胞淋巴结微转移方面的应用价值。方法:以胃周围淋巴结为检测标本,用反转录套式聚合酶链反应技术(RT-NP-PCR)检测CEA mRNA,用常规病理学方法检查癌细胞。结果:胃癌组胃周围淋巴结38.8%CEA mRNA阳性,病理学检测17.9%癌细胞阳性;良性胃病组胃周围淋巴结均阴性。RT-NP-PCR检测CEA mRNA与病理学检查癌细胞的结果比较,有非常显著性差异(P<0.01)。结论:CEA mRNA指标诊断胃癌细胞淋巴结微转移的敏感性较高,具有较高的应用价值,可以在病理学诊断胃癌淋巴结转移时开展应用。
Objective： To acquaint the applied value on carcinoembryonic antigen （CEA） mRNA being detected in lymph node to diagnose gastric cancer cells micrometastasis. Methods： The detected specimens were the lymph nodes nearby stomach in the study. CEA mRNA in the specimens was detected by reverse transcriptase-nested primers-polymerase chain reaction （RT-NP-PCR）. Cancer cells were detected by routine pathologic method. Results： In the gastric cancer group, the positive results of CEA mRNA were 26 （38.8%） and the positive results of cancer cells were 12 （17.9%） in the lymph nodes from 67 patients with gastric cancer. In the control group, the detected results were all negative in lymph nodes from 21 patients with benign gastric disease. The difference positive results were significant （P〈0.01） between the RT-NP-PCR and pathologic method in identifying the micrometastasis lymph node. Conclusion： The sensitivity of CEA mRNA is higher than cancer cells by routine pathologic method on the diagnosis of gastric cancer cells micrometastasis, and CEA mRNA is a useful index to diagnose the micrometastasis.
出处
《中国医药导报》
CAS
2007年第08Z期13-14,共2页
China Medical Herald
关键词
胃癌
癌胚抗原信使核糖核酸
淋巴结
癌细胞微转移
Gastric cancer
Carcinoembryonic antigen mRNA
Lymph node
Cancer cells micrometastasis
