摘要
利用新型3',6'-对戊酰硼荧光素探针,结合超氧化物歧化酶(SOD)的选择性催化歧化反应特性,建立了一种超氧阴离子(O2-)的酶催化荧光分析方法。SOD催化黄嘌呤/黄嘌呤氧化酶体系中的O2-生成H2O2,使得荧光探针转换为强荧光的荧光素产物,从而实现对O2-的荧光检测。该方法检测线性范围为2.4×10-7～1.5×10-5mol/L,检测下限1.0×10-7mol/L,显示了较高选择性和灵敏度。
A enzymatic assay for superoxide anion （02^- ） has been developed by using a new H2O2 fluorescent probe, 3＇, 6＇ - bis（pinacolatoboron） fluoran, as the fluorescent substrate of superoxide dismutase （SOD） . The superoxide anion in xanthine/xanthine oxidase system was catalyzed by SOD to form H2O2, resulting in a clear increase in the fluorescence intensity of the fluorescent probe. The experimental results show that there is a linear relationship between the fluorescence intensity and the concentration of superoxide anion in the range of 2.4 x 10^-7 1.5 x 10^-5 mol/L and the detection limit reaches 1.0 x 10^-7 mol/L.
出处
《化学传感器》
CAS
2007年第2期42-46,共5页
Chemical Sensors
基金
国家自然科学基金(No.20205004)
教育部科学技术研究重点项目(No.106122)资助
