摘要
为了研究蜂胶醇提物在细胞内的抗氧化活性(CAA),本实验将2′,7′-二氯荧光二乙酸盐(DCFH-DA)荧光探针装载到人肝癌HepG2细胞内,然后被进入到细胞内的2,2′-偶氮二异丁基脒二盐酸盐(ABAP)产生的活性氧氧化成有荧光的二氯荧光(DCF);以荧光值为指标,使用多功能酶标仪和荧光倒置显微镜分析蜂胶醇提物清除活性氧的能力。结果表明:蜂胶醇提物具有明显的抗氧化活性,并呈现良好的剂量-效应关系。当CAA值达到50时,蜂胶醇提物质量浓度仅0.14mg/mL;当蜂胶醇提物质量浓度为0.5mg/mL时,细胞内的活性氧显著减少,有绿色荧光的细胞数几乎减少一半,清除率接近50%。蜂胶醇提物具有非常好的细胞内抗氧化能力。
A cellular antioxidant activity （CAA） assay for quantifying the antioxidant activity of an ethanol extract from propolis （EEP） provided by Bee Research Institute,Chinese Academy of Agricultural Sciences has been developed. Dichlorofluorescein-diacetate （DCFH-DA） as a probe,which is easily oxidized into fluorescent dichlorofluorescein （DCF） by reactive oxygen species （ROS）,was trapped within cells and the method was used to measure the ability of EEP to prevent the formation of DCF by 2,2＇-azobis（2-amidinopropane） dihydrochloride （ABAP）-generated peroxyl radicals in human liver carcinoma HepG2 cells with multi-functional microplate reader and inverted fluorescence microscope. The decrease in cellular fluorescence intensity compared to the control cells indicates the antioxidant capacity of EEP. In addition,this bioactivity depended on dose. The concentration of EEP corresponding to CAA unit50 was only 0.14 mg/mL. A ROS clearance rate of 47.05% was obtained at an EEP concentration of 0.5 mg/mL.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第19期190-193,共4页
Food Science
基金
国家现代农业(蜂)产业技术体系建设专项(NYCYTX-43)
农业部公益性行业(农业)科研专项(nyhyzx07-041)
