摘要
从八种大孔树脂中筛选出了适宜纯化核桃叶黄酮的大孔树脂,并对纯化后核桃叶黄酮的细胞内抗氧化活性、对羟自由基清除力、对DPPH自由基清除力和对超氧阴离子自由基清除力进行了研究。结果表明,AB-8型大孔树脂的纯化效果最佳,核桃叶黄酮具有明显的抗氧化活性。核桃叶黄酮对羟自由基清除率高于VC,对超氧阴离子自由基抑制率和DPPH自由基清除率低于Vc,其IC50为22.4μg/mL。核桃叶黄酮在结肠癌细胞(HT-29)中表现出明显的抗氧化活性,其抗氧化性随浓度递增。
The adsorption and desorption of eight macroporous resins for flavonoids from Juglans regia leaves were compared.The optimal adsorbent was AB-8.Moreover,the ability of purified flavonoidson cellular antioxidant,scavenging hydroxyl radical （· OH）,scavenging 1-diphenyl-2-picrylhydrazy （DPPH） and superoxide anion radical （62-） was investigated.The results showed that flavonoids have good antioxidant activity.Compared with VC,the flavonoids from Juglans regia leaves had stronger capacity of scavenging on OH and 62-,but weaker capacity of scavenging on DPPH with IC5o 0.0224mg/mL.Furthermore,the flavonoids showed obvious antioxidant activity in colon cancer cell （HT-29）,and it was concentration-dependent.
出处
《中国食品添加剂》
CAS
北大核心
2013年第6期209-216,共8页
China Food Additives
基金
天津市自然科学基金重点项目(120029)
天津市高校科技发展基金计划项目(20110608)
关键词
核桃叶黄酮
纯化
抗氧化
细胞模型
结肠癌细胞
Juglans regia leaves flavonoids
purification
antioxidant activity cell model
colon cancer cell
