摘要
[目的]研究HSP70过表达对紫外线致DNA损伤的影响。[方法]设A549、A549／pcDNA、A549/HSP703个组,分别用不同紫外线照射剂量(0、10、20、40、80J／m2)照射HSP70表达正常和过表达的A549细胞株,用Western-blot检测HSP70的水平,用碱性单细胞凝胶电泳检测DNA损伤情况。[结果]DNA损伤的彗星率随紫外线照射的剂量增加而逐渐加重。当在相同剂量40J/m2时,A549/HSP70组的彗星率为24％、尾矩为14．31±1．60,A549相应为40％、18．83±0．41, A549／pcDNA为40％、18．67±0．81。在40J／m2照射所致的Olive尾矩明显减少,与对照组比较差异有显著性(P<0．01)。[结论]HSP70能保护细胞免受一定剂量范围内的紫外线引起的DNA损伤。
[ Objective ] To study the protective role of HSP70 against DNA damage caused hy ultraviolet C. [ Methods ] A549 cells transfected hy HSP70 gene were irradiated hy different strength of Ultraviolet C（ 0, 10, 20, 40, 80J/m^2 ）, with A549 cells as normal control and A549 cells trasfected hy pcDNA3.0 vector as a blank control. HSP70 expression was detected using western-blot assay. DNA damage was detected using single cell gel electrophoresis（ SCGE ）. [ Results ] The results showed that the levels of DNA damage increased with the increasing strength of Ultraviolet C in A549 and transfected A549 cells. The protection against UVC-induced DNA damage in cells with overexpressed HSP70 was observed at UVC close 20J/m2 and 40J/m2. Compared with A549 cells, the values of the Olive tail moment in cells with overexpressed HSP70 decreased significantly （ P 〈 0.01 ）. [ Conclusion ] These results suggested that overexpressed HSP70 may play a role in protecting A549 cells from DNA damage caused hy UVC irradiation and that there is a threshold of protection of HSP70 from DNA damage hy UVC irradiation.
出处
《环境与职业医学》
CAS
北大核心
2007年第2期186-189,共4页
Journal of Environmental and Occupational Medicine
基金
国家"973"重大基础研究项目(编号:2002CB512905)
国家自然科学基金项目(编号:30371204)
